This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Actis, L A Articles by Crosa, J H Search for Related Content PubMed PubMed Citation Articles by Actis, L A Articles by Crosa, J H

Iron-regulated outer membrane protein OM2 of Vibrio anguillarum is encoded by virulence plasmid pJM1.

ABSTRACT

Vibrio anguillarum 775 harboring the virulence plasmid pJM1 synthesized an outer membrane protein of 86 kilodaltons, OM2, that was inducible under conditions of iron limitation. pJM1 DNA fragments obtained by digestion with restriction endonucleases were cloned into cosmid vectors and transferred into Escherichia coli. The OM2 protein was synthesized in E. coli, demonstrating that it is actually encoded by the pJM1 plasmid. Mobilization of the recombinant plasmids to V. anguillarum was accomplished by using the transfer factor pRK2013. A V. anguillarum exconjugant harboring the recombinant derivative pJHC-T7 and synthesizing the OM2 protein took up 55Fe3+ and grew under iron-limiting conditions, only in presence of the pJM1-mediated siderophore. Exconjugants harboring recombinant plasmids, such as pJHC-T2 which did not encode the OM2 protein, were transport negative. Membrane protein iodination experiments, together with protease treatment of whole cells, indicated that the OM2 protein is exposed to the outside environment of the V. anguillarum cells.

This article has been cited by other articles:

• Stork, M., Otto, B. R., Crosa, J. H. (2007). A Novel Protein, TtpC, Is a Required Component of the TonB2 Complex for Specific Iron Transport in the Pathogens Vibrio anguillarum and Vibrio cholerae. J. Bacteriol. 189: 1803-1815 [Abstract] [Full Text]  
• Rhodes, E. R., Shoemaker, C. J., Menke, S. M., Edelmann, R. E., Actis, L. A. (2007). Evaluation of different iron sources and their influence in biofilm formation by the dental pathogen Actinobacillus actinomycetemcomitans. J Med Microbiol 56: 119-128 [Abstract] [Full Text]  
• Welch, T. J., Crosa, J. H. (2005). Novel Role of the Lipopolysaccharide O1 Side Chain in Ferric Siderophore Transport and Virulence of Vibrio anguillarum. Infect. Immun. 73: 5864-5872 [Abstract] [Full Text]  
• Alice, A. F., Lopez, C. S., Crosa, J. H. (2005). Plasmid- and Chromosome-Encoded Redundant and Specific Functions Are Involved in Biosynthesis of the Siderophore Anguibactin in Vibrio anguillarum 775: a Case of Chance and Necessity?. J. Bacteriol. 187: 2209-2214 [Abstract] [Full Text]  
• Stork, M., Di Lorenzo, M., Mourino, S., Osorio, C. R., Lemos, M. L., Crosa, J. H. (2004). Two tonB Systems Function in Iron Transport in Vibrio anguillarum, but Only One Is Essential for Virulence. Infect. Immun. 72: 7326-7329 [Abstract] [Full Text]  
• Di Lorenzo, M., Poppelaars, S., Stork, M., Nagasawa, M., Tolmasky, M. E., Crosa, J. H. (2004). A Nonribosomal Peptide Synthetase with a Novel Domain Organization Is Essential for Siderophore Biosynthesis in Vibrio anguillarum. J. Bacteriol. 186: 7327-7336 [Abstract] [Full Text]  
• Dorsey, C. W., Tomaras, A. P., Connerly, P. L., Tolmasky, M. E., Crosa, J. H., Actis, L. A. (2004). The siderophore-mediated iron acquisition systems of Acinetobacter baumannii ATCC 19606 and Vibrio anguillarum 775 are structurally and functionally related. Microbiology 150: 3657-3667 [Abstract] [Full Text]  
• Tall, B. D., Fall, S., Pereira, M. R., Ramos-Valle, M., Curtis, S. K., Kothary, M. H., Chu, D. M. T., Monday, S. R., Kornegay, L., Donkar, T., Prince, D., Thunberg, R. L., Shangraw, K. A., Hanes, D. E., Khambaty, F. M., Lampel, K. A., Bier, J. W., Bayer, R. C. (2003). Characterization of Vibrio fluvialis-Like Strains Implicated in Limp Lobster Disease. Appl. Environ. Microbiol. 69: 7435-7446 [Abstract] [Full Text]  
• Di Lorenzo, M., Stork, M., Tolmasky, M. E., Actis, L. A., Farrell, D., Welch, T. J., Crosa, L. M., Wertheimer, A. M., Chen, Q., Salinas, P., Waldbeser, L., Crosa, J. H. (2003). Complete Sequence of Virulence Plasmid pJM1 from the Marine Fish Pathogen Vibrio anguillarum Strain 775. J. Bacteriol. 185: 5822-5830 [Abstract] [Full Text]  
• Dorsey, C. W., Beglin, M. S., Actis, L. A. (2003). Detection and Analysis of Iron Uptake Components Expressed by Acinetobacter baumannii Clinical Isolates. J. Clin. Microbiol. 41: 4188-4193 [Abstract] [Full Text]  
• Dorsey, C. W., Tolmasky, M. E., Crosa, J. H., Actis, L. A. (2003). Genetic organization of an Acinetobacter baumannii chromosomal region harbouring genes related to siderophore biosynthesis and transport. Microbiology 149: 1227-1238 [Abstract] [Full Text]  
• Dorsey, C. W., Tomaras, A. P., Actis, L. A. (2002). Genetic and Phenotypic Analysis of Acinetobacter baumannii Insertion Derivatives Generated with a Transposome System. Appl. Environ. Microbiol. 68: 6353-6360 [Abstract] [Full Text]  
• Crosa, J. H., Walsh, C. T. (2002). Genetics and Assembly Line Enzymology of Siderophore Biosynthesis in Bacteria. Microbiol. Mol. Biol. Rev. 66: 223-249 [Abstract] [Full Text]  
• Okuda, J., Nakai, T., Chang, P. S., Oh, T., Nishino, T., Koitabashi, T., Nishibuchi, M. (2001). The toxR Gene of Vibrio (Listonella) anguillarum Controls Expression of the Major Outer Membrane Proteins but Not Virulence in a Natural Host Model. Infect. Immun. 69: 6091-6101 [Abstract] [Full Text]  
• Echenique, J. R., Dorsey, C. W., Patrito, L. C., Petroni, A., Tolmasky, M. E., Actis, L. A. (2001). Acinetobacter baumannii has two genes encoding glutathione-dependent formaldehyde dehydrogenase: evidence for differential regulation in response to iron. Microbiology 147: 2805-2815 [Abstract] [Full Text]  
• Welch, T. J., Chai, S., Crosa, J. H. (2000). The Overlapping angB and angG Genes Are Encoded within the trans-Acting Factor Region of the Virulence Plasmid in Vibrio anguillarum: Essential Role in Siderophore Biosynthesis. J. Bacteriol. 182: 6762-6773 [Abstract] [Full Text]  
• Wang, X. H., Leung, K. Y. (2000). Biochemical characterization of different types of adherence of Vibrio species to fish epithelial cells. Microbiology 146: 989-998 [Abstract] [Full Text]  
• Wertheimer, A. M., Verweij, W., Chen, Q., Crosa, L. M., Nagasawa, M., Tolmasky, M. E., Actis, L. A., Crosa, J. H. (1999). Characterization of the angR Gene of Vibrio anguillarum: Essential Role in Virulence. Infect. Immun. 67: 6496-6509 [Abstract] [Full Text]  
• Chai, S., Welch, T. J., Crosa, J. H. (1998). Characterization of the Interaction between Fur and the Iron Transport Promoter of the Virulence Plasmid in Vibrio anguillarum. J. Biol. Chem. 273: 33841-33847 [Abstract] [Full Text]  
• Litwin, C. M., Byrne, B. L. (1998). Cloning and Characterization of an Outer Membrane Protein of Vibrio vulnificus Required for Heme Utilization: Regulation of Expression and Determination of the Gene Sequence. Infect. Immun. 66: 3134-3141 [Abstract] [Full Text]  
• Chen, Q., Crosa, J. H. (1996). Antisense RNA, Fur, Iron, and the Regulation of Iron Transport Genes in Vibrio anguillarum. J. Biol. Chem. 271: 18885-18891 [Abstract] [Full Text]