This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ramakrishnan, G Articles by Inouye, M Search for Related Content PubMed PubMed Citation Articles by Ramakrishnan, G Articles by Inouye, M

Uncoupling of osmoregulation of the Escherichia coli K-12 ompF gene from ompB-dependent transcription.

ABSTRACT

The expression of the genes for the Escherichia coli K-12 outer membrane proteins, ompF and ompC, is subject to osmoregulation and responds to changes in the osmolarity of the growth medium. The transcription of these genes is dependent on the products of the regulatory locus ompB (comprising the genes ompR and envZ). The native promoter of ompF was replaced with an inducible lpp promoter to eliminate this transcriptional dependence of ompF expression on ompB. As a result, it was possible for the OmpF protein to be produced in an ompB mutant strain that does not normally express ompF. Surprisingly, the expression of ompF under the lpp promoter was still osmoregulated not only in the ompB+ strain but also in two ompB strains tested. These results indicate the involvement of a factor(s) besides the ompR and envZ gene products in the osmoregulation of OmpF production. This factor may interact with a sequence downstream of the ompF promoter. In addition, we show that the expression of ompF under the lpp promoter has no direct effect on ompC expression.

This article has been cited by other articles:

• Batchelor, E., Silhavy, T. J., Goulian, M. (2004). Continuous Control in Bacterial Regulatory Circuits. J. Bacteriol. 186: 7618-7625 [Abstract] [Full Text]  
• Prilipov, A., Phale, P. S., Koebnik, R., Widmer, C., Rosenbusch, J. P. (1998). Identification and Characterization of Two Quiescent Porin Genes, nmpC and ompN, in Escherichia coli BE. J. Bacteriol. 180: 3388-3392 [Abstract] [Full Text]  
• Mecsas, J, Rouviere, P E, Erickson, J W, Donohue, T J, Gross, C A (1993). The activity of sigma E, an Escherichia coli heat-inducible sigma-factor, is modulated by expression of outer membrane proteins.. Genes Dev. 7: 2618-2628 [Abstract]