This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ballantine, S P Articles by Boxer, D H Search for Related Content PubMed PubMed Citation Articles by Ballantine, S P Articles by Boxer, D H

Nickel-containing hydrogenase isoenzymes from anaerobically grown Escherichia coli K-12.

ABSTRACT

Two membrane-bound hydrogenase isoenzymes present in Escherichia coli during anaerobic growth have been resolved. The isoenzymes are immunologically and electrophoretically distinct. The physically more abundant isoenzyme (hydrogenase 1) contains a subunit of Mr 64,000 and is not released from the membrane by exposure to either trypsin or pancreatin. The second isoenzyme (hydrogenase 2) apparently contributes the greater part of the membrane-bound hydrogen:benzyl viologen oxidoreductase activity and exists in two electrophoretic forms revealed by nondenaturing polyacrylamide gel analysis. This isoenzyme is irreversibly inactivated at alkaline pH and gives rise to an active, soluble derivative when the membrane-bound enzyme is exposed to either trypsin or pancreatin. Both hydrogenase isoenzymes contain nickel.

This article has been cited by other articles:

• Zhang, J. W., Leach, M. R., Zamble, D. B. (2007). The Peptidyl-Prolyl Isomerase Activity of SlyD Is Not Required for Maturation of Escherichia coli Hydrogenase. J. Bacteriol. 189: 7942-7944 [Abstract] [Full Text]  
• Park, M.-O., Mizutani, T., Jones, P. R. (2007). Glyceraldehyde-3-Phosphate Ferredoxin Oxidoreductase from Methanococcus maripaludis. J. Bacteriol. 189: 7281-7289 [Abstract] [Full Text]  
• Benanti, E. L., Chivers, P. T. (2007). The N-terminal Arm of the Helicobacter pylori Ni2+-dependent Transcription Factor NikR Is Required for Specific DNA Binding. J. Biol. Chem. 282: 20365-20375 [Abstract] [Full Text]  
• Leach, M. R., Zhang, J. W., Zamble, D. B. (2007). The Role of Complex Formation between the Escherichia coli Hydrogenase Accessory Factors HypB and SlyD. J. Biol. Chem. 282: 16177-16186 [Abstract] [Full Text]  
• Yang, F., Hu, W., Xu, H., Li, C., Xia, B., Jin, C. (2007). Solution Structure and Backbone Dynamics of an Endopeptidase HycI from scherichia coli: IMPLICATIONS FOR MECHANISM OF THE [NiFe] HYDROGENASE MATURATION. J. Biol. Chem. 282: 3856-3863 [Abstract] [Full Text]  
• Rowe, J. L., Starnes, G. L., Chivers, P. T. (2005). Complex Transcriptional Control Links NikABCDE-Dependent Nickel Transport with Hydrogenase Expression in Escherichia coli. J. Bacteriol. 187: 6317-6323 [Abstract] [Full Text]  
• Zhang, J. W., Butland, G., Greenblatt, J. F., Emili, A., Zamble, D. B. (2005). A Role for SlyD in the Escherichia coli Hydrogenase Biosynthetic Pathway. J. Biol. Chem. 280: 4360-4366 [Abstract] [Full Text]  
• Blokesch, M., Rohrmoser, M., Rode, S., Bock, A. (2004). HybF, a Zinc-Containing Protein Involved in NiFe Hydrogenase Maturation. J. Bacteriol. 186: 2603-2611 [Abstract] [Full Text]  
• Hube, M., Blokesch, M., Bock, A. (2002). Network of Hydrogenase Maturation in Escherichia coli: Role of Accessory Proteins HypA and HybF. J. Bacteriol. 184: 3879-3885 [Abstract] [Full Text]  
• Fodor, B., Rákhely, G., Kovács, A. T., Kovács, K. L. (2001). Transposon Mutagenesis in Purple Sulfur Photosynthetic Bacteria: Identification of hypF, Encoding a Protein Capable of Processing [NiFe] Hydrogenases in {alpha}, {beta}, and {gamma} Subdivisions of the Proteobacteria. Appl. Environ. Microbiol. 67: 2476-2483 [Abstract] [Full Text]  
• Blokesch, M., Magalon, A., Böck, A. (2001). Interplay between the Specific Chaperone-Like Proteins HybG and HypC in Maturation of Hydrogenases 1, 2, and 3 from Escherichia coli. J. Bacteriol. 183: 2817-2822 [Abstract] [Full Text]  
• Sargent, F., Stanley, N. R., Berks, B. C., Palmer, T. (1999). Sec-independent Protein Translocation in Escherichia coli. A DISTINCT AND PIVOTAL ROLE FOR THE TatB PROTEIN. J. Biol. Chem. 274: 36073-36082 [Abstract] [Full Text]  
• Richard, D. J., Sawers, G., Sargent, F., McWalter, L., Boxer, D. H. (1999). Transcriptional regulation in response to oxygen and nitrate of the operons encoding the [NiFe] hydrogenases 1 and 2 of Escherichia coli. Microbiology 145: 2903-2912 [Abstract] [Full Text]  
• Bogsch, E. G., Sargent, F., Stanley, N. R., Berks, B. C., Robinson, C., Palmer, T. (1998). An Essential Component of a Novel Bacterial Protein Export System with Homologues in Plastids and Mitochondria. J. Biol. Chem. 273: 18003-18006 [Abstract] [Full Text]  
• Magalon, A., Bock, A. (2000). Analysis of the HypC-HycE Complex, a Key Intermediate in the Assembly of the Metal Center of the Escherichia coli Hydrogenase 3. J. Biol. Chem. 275: 21114-21120 [Abstract] [Full Text]