This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kokjohn, T A Articles by Miller, R V Search for Related Content PubMed PubMed Citation Articles by Kokjohn, T A Articles by Miller, R V

Molecular cloning and characterization of the recA gene of Pseudomonas aeruginosa PAO.

ABSTRACT

The recA gene of Pseudomonas aeruginosa PAO has been isolated and introduced into Escherichia coli K-12. Resistance to killing by UV irradiation was restored in several RecA-E. coli K-12 hosts by the P. aeruginosa gene, as was resistance to methyl methanesulfonate. Recombination proficiency was also restored, as measured by HfrH-mediated conjugation and by the ability to propagate Fec-phage lambda derivatives. The cloned P. aeruginosa recA gene restored both spontaneous and mitomycin C-stimulated induction of lambda prophage in lysogens of a recA strain of E. coli K-12.

This article has been cited by other articles:

• Fernandez Zenoff, V., Sineriz, F., Farias, M. E. (2006). Diverse Responses to UV-B Radiation and Repair Mechanisms of Bacteria Isolated from High-Altitude Aquatic Environments. Appl. Environ. Microbiol. 72: 7857-7863 [Abstract] [Full Text]  
• Elasri, M. O., Miller, R. V. (1999). Study of the Response of a Biofilm Bacterial Community to UV Radiation. Appl. Environ. Microbiol. 65: 2025-2031 [Abstract] [Full Text]