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J Bacteriol. 1985 August; 163(2): 735-737

Protoplast water content of bacterial spores determined by buoyant density sedimentation.

J A Lindsay, T C Beaman and P Gerhardt

ABSTRACT

Protoplast wet densities (1.315 to 1.400 g/ml), determined by buoyant density sedimentation in Metrizamide gradients, were correlated inversely with the protoplast water contents (26.4 to 55.0 g of water/100 g of wet protoplast) of nine diverse types of pure lysozyme-sensitive dormant bacterial spores. The correlation equation provided a precise method for obtaining the protoplast water contents of other spore types with small impure samples and indicated that the average protoplast dry density was 1.460 g/ml.

J Bacteriol. 1985 August; 163(2): 735-737




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Appl. Environ. Microbiol. Infect. Immun. Eukaryot. Cell
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