This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bishop, L J Articles by Cramer, W A Search for Related Content PubMed PubMed Citation Articles by Bishop, L J Articles by Cramer, W A

Localization of the immunity protein-reactive domain in unmodified and chemically modified COOH-terminal peptides of colicin E1.

ABSTRACT

The region of the colicin E1 polypeptide that interacts with immunity protein has been localized to a 168-residue COOH-terminal peptide. This is the length of a proteolytically generated peptide fragment of colicin E1 against which imm+ function can be demonstrated in osmotically shocked cells. The role of particular amino acids of the COOH-terminal peptide in the expression of the immune phenotype was studied. Chemical modification showed that the two histidine residues (His 427 and His 440) and the single cysteine residue (Cys 505) present in the COOH-terminal peptide were not necessary for the colicin-immunity protein interaction. The immunity protein was localized in the cytoplasmic membrane fraction, consistent with previous work of others on the colicin Ia immunity protein and the prediction from the immunity protein amino acid sequence that it is a hydrophobic protein. The distribution of hydrophobic residues along the immunity polypeptide was calculated.

This article has been cited by other articles:

• Cascales, E., Buchanan, S. K., Duche, D., Kleanthous, C., Lloubes, R., Postle, K., Riley, M., Slatin, S., Cavard, D. (2007). Colicin Biology. Microbiol. Mol. Biol. Rev. 71: 158-229 [Abstract] [Full Text]  
• Sobko, A. A., Kotova, E. A., Antonenko, Y. N., Zakharov, S. D., Cramer, W. A. (2006). Lipid Dependence of the Channel Properties of a Colicin E1-Lipid Toroidal Pore. J. Biol. Chem. 281: 14408-14416 [Abstract] [Full Text]  
• Zakharov, S. D., Rokitskaya, T. I., Shapovalov, V. L., Antonenko, Y. N., Cramer, W. A. (2002). Tuning the membrane surface potential for efficient toxin import. Proc. Natl. Acad. Sci. USA 99: 8654-8659 [Abstract] [Full Text]  
• Lindeberg, M., Cramer, W. A. (2001). Identification of Specific Residues in Colicin E1 Involved in Immunity Protein Recognition. J. Bacteriol. 183: 2132-2136 [Abstract] [Full Text]  
• Zakharov, S. D., Lindeberg, M., Griko, Y., Salamon, Z., Tollin, G., Prendergast, F. G., Cramer, W. A. (1998). Membrane-bound state of the colicin E1 channel domain as an extended two-dimensional helical array. Proc. Natl. Acad. Sci. USA 95: 4282-4287 [Abstract] [Full Text]