This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Jovanovich, S B Articles by Lebowitz, J Search for Related Content PubMed PubMed Citation Articles by Jovanovich, S B Articles by Lebowitz, J

 Previous Article  |  Next Article 

J Bacteriol. 1987 October; 169(10): 4431-4435

Estimation of the effect of coumermycin A1 on Salmonella typhimurium promoters by using random operon fusions.

Department of Microbiology, University of Alabama, Birmingham 35294.

ABSTRACT

We have estimated the extent to which relaxation of supercoiling by the DNA gyrase inhibitor coumermycin A1 affects gene expression in vivo in Salmonella typhimurium. We isolated a set of Mu d1-8 Lac+ operon fusions to random promoters and measured the effect of coumermycin A1 on the expression of 67 fusions. The differential rate of synthesis was increased for 70% of the fusions and decreased for 16%, and 13% of the fusions had less than a 25% change in expression. The coumermycin A1 response was found to correlate well (P = 0.067) with the basal level of expression such that coumermycin A1 tended to stimulate fusions with low expression and inhibit those with high expression. Since the vast majority of the fusions were sensitive to coumermycin A1 addition and, therefore, to the level of supercoiling, these results indicate that if the level of supercoiling were to vary under physiological conditions, then major readjustments in the cellular economy would occur.

This article has been cited by other articles:

• Schmitz, G. E., Downs, D. M. (2006). An Allele of gyrA Prevents Salmonella enterica Serovar Typhimurium from Using Succinate as a Carbon Source.. J. Bacteriol. 188: 3126-3129 [Abstract] [Full Text]  
• Crozat, E., Philippe, N., Lenski, R. E., Geiselmann, J., Schneider, D. (2005). Long-Term Experimental Evolution in Escherichia coli. XII. DNA Topology as a Key Target of Selection. Genetics 169: 523-532 [Abstract] [Full Text]  
• Gmuender, H., Kuratli, K., Di Padova, K., Gray, C. P., Keck, W., Evers, S. (2001). Gene Expression Changes Triggered by Exposure of Haemophilus influenzae to Novobiocin or Ciprofloxacin: Combined Transcription and Translation Analysis. Genome Res 11: 28-42 [Abstract] [Full Text]