This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Su, C J Articles by Sadoff, H L Search for Related Content PubMed PubMed Citation Articles by Su, C J Articles by Sadoff, H L

 Previous Article  |  Next Article 

J Bacteriol. 1987 October; 169(10): 4451-4456

Protein synthesis during encystment of Azotobacter vinelandii.

Department of Microbiology and Public Health, Michigan State University, East Lansing 48824-1101.

ABSTRACT

Proteins synthesized during the encystment of Azotobacter vinelandii were radiolabeled with [35S]methionine and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Pulse labeling was used to demonstrate that early encystment-specific proteins were beginning to be synthesized at 2 h and reached peak levels about 12 h after initiation of encystment. One such protein was identified as a beta-ketoacyl acyl-carrier protein synthase. The concentration of early proteins began to decrease at 16 h, when intermediate proteins specific to the differentiation process began to be synthesized. The cessation of synthesis of intermediate proteins began at 20 h postinitiation, and the labeling pattern of proteins then remained constant throughout the remaining 4 days of encystment.