This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tomenchok, D M
Right arrow Articles by Brandriss, M C
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tomenchok, D M
Right arrow Articles by Brandriss, M C

 Previous Article  |  Next Article 

J Bacteriol. 1987 December; 169(12): 5364-5372

Gene-enzyme relationships in the proline biosynthetic pathway of Saccharomyces cerevisiae.

D M Tomenchok and M C Brandriss

Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey-New Jersey Medical School, Newark 07103.

ABSTRACT

The PRO1, PRO2, and PRO3 genes were isolated by functional complementation of pro1, pro2, and pro3 (proline-requiring) strains of Saccharomyces cerevisiae. Independent clones with overlapping inserts were isolated from S. cerevisiae genomic libraries in YEp24 (2 microns) and YCp50 (CEN) plasmids. The identity of each gene was determined by gene disruption, and Southern hybridization and genetic analyses confirmed that the bona fide genes had been cloned. Plasmids containing each gene were introduced into known bacterial proline auxotrophs, and the ability to restore proline prototrophy was assessed. Interspecies complementation demonstrated that the S. cerevisiae PRO1 gene encoded gamma-glutamyl kinase, PRO2 encoded gamma-glutamyl phosphate reductase, and PRO3 encoded delta 1-pyrroline-5-carboxylate reductase. The presence of the PRO3 gene on a high-copy-number plasmid in S. cerevisiae caused a 20-fold overproduction of delta 1-pyrroline-5-carboxylate reductase. The PRO2 gene mapped on chromosome XV tightly linked to cdc66, and the PRO3 gene was located on the right arm of chromosome V between HIS1 and the centromere.

J Bacteriol. 1987 December; 169(12): 5364-5372




This article has been cited by other articles:

  • Kaino, T., Tateiwa, T., Mizukami-Murata, S., Shima, J., Takagi, H. (2008). Self-Cloning Baker's Yeasts That Accumulate Proline Enhance Freeze Tolerance in Doughs. Appl. Environ. Microbiol. 74: 5845-5849 [Abstract] [Full Text]  
  • Sekine, T., Kawaguchi, A., Hamano, Y., Takagi, H. (2007). Desensitization of Feedback Inhibition of the Saccharomyces cerevisiae {gamma}-Glutamyl Kinase Enhances Proline Accumulation and Freezing Tolerance. Appl. Environ. Microbiol. 73: 4011-4019 [Abstract] [Full Text]  
  • Takagi, H., Takaoka, M., Kawaguchi, A., Kubo, Y. (2005). Effect of L-Proline on Sake Brewing and Ethanol Stress in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 71: 8656-8662 [Abstract] [Full Text]  
  • Nomura, M., Takagi, H. (2004). Role of the yeast acetyltransferase Mpr1 in oxidative stress: Regulation of oxygen reactive species caused by a toxic proline catabolism intermediate. Proc. Natl. Acad. Sci. USA 101: 12616-12621 [Abstract] [Full Text]  
  • Terao, Y., Nakamori, S., Takagi, H. (2003). Gene Dosage Effect of L-Proline Biosynthetic Enzymes on L-Proline Accumulation and Freeze Tolerance in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 69: 6527-6532 [Abstract] [Full Text]  
  • Martin, O., Brandriss, M. C., Schneider, G., Bakalinsky, A. T. (2003). Improved Anaerobic Use of Arginine by Saccharomyces cerevisiae. Appl. Environ. Microbiol. 69: 1623-1628 [Abstract] [Full Text]  
  • Morita, Y., Nakamori, S., Takagi, H. (2003). L-Proline Accumulation and Freeze Tolerance in Saccharomyces cerevisiae Are Caused by a Mutation in the PRO1 Gene Encoding {gamma}-Glutamyl Kinase. Appl. Environ. Microbiol. 69: 212-219 [Abstract] [Full Text]  
  • Takagi, H., Shichiri, M., Takemura, M., Mohri, M., Nakamori, S. (2000). Saccharomyces cerevisiae Sigma 1278b Has Novel Genes of the N-Acetyltransferase Gene Superfamily Required for L-Proline Analogue Resistance. J. Bacteriol. 182: 4249-4256 [Abstract] [Full Text]  
  • Patrick, M., Bradley, T. (2000). The physiology of salinity tolerance in larvae of two species of Culex mosquitoes: the role of compatible solutes. J. Exp. Biol. 203: 821-830 [Abstract]  
  • Hu, C.-a. A., Lin, W.-W., Obie, C., Valle, D. (1999). Molecular Enzymology of Mammalian Delta 1-Pyrroline-5-carboxylate Synthase. ALTERNATIVE SPLICE DONOR UTILIZATION GENERATES ISOFORMS WITH DIFFERENT SENSITIVITY TO ORNITHINE INHIBITION. J. Biol. Chem. 274: 6754-6762 [Abstract] [Full Text]  
  • Spector, D., Labarre, J., Toledano, M. B. (2001). A Genetic Investigation of the Essential Role of Glutathione. MUTATIONS IN THE PROLINE BIOSYNTHESIS PATHWAY ARE THE ONLY SUPPRESSORS OF GLUTATHIONE AUXOTROPHY IN YEAST. J. Biol. Chem. 276: 7011-7016 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»