This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Salminen, S O Articles by Streeter, J G Search for Related Content PubMed PubMed Citation Articles by Salminen, S O Articles by Streeter, J G

 Previous Article  |  Next Article 

J Bacteriol. 1987 February; 169(2): 495-499

Involvement of glutamate in the respiratory metabolism of Bradyrhizobium japonicum bacteroids.

ABSTRACT

Bradyrhizobium japonicum bacteroids were isolated anaerobically and supplied with 14C-labeled succinate, malate, aspartate, or glutamate for periods of up to 60 min in the presence of myoglobin to control the O2 concentration. Succinate and malate were absorbed about twice as rapidly as glutamate and aspartate. Conversion of substrate to CO2 was most rapid for malate, followed by succinate, glutamate, and aspartate. When CO2 production was expressed as a proportion of total carbon taken up, malate was still the most rapidly respired substrate, with 68% of the label absorbed converted to CO2. The comparable values for succinate, glutamate, and aspartate were 37, 50, and 38%, respectively. Considering the fate of labeled substrate not respired, greater than 95% of absorbed glutamate remained as glutamate in the bacteroids. In contrast, from 39 to 66% of the absorbed succinate, malate, or aspartate was converted to glutamate. An increase in the rate of CO2 formation from labeled substrates after 20 min appeared to coincide with a maximum accumulation of label in glutamate. The results indicate the presence of a substantial glutamate pool in bacteroids and the involvement of glutamate in the respiratory metabolism of bacteroids.

This article has been cited by other articles:

• Castillo, A., Taboada, H., Mendoza, A., Valderrama, B., Encarnación, S., Mora, J. (2000). Role of GOGAT in carbon and nitrogen partitioning in Rhizobium etli. Microbiology 146: 1627-1637 [Abstract] [Full Text]  
• SANGWAN, I., O'BRIAN, M. R. (1991). Evidence for an Inter-Organismic Heme Biosynthetic Pathway in Symbiotic Soybean Root Nodules. Science 251: 1220-1222 [Abstract]