This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ohnishi, K Articles by Iino, T Search for Related Content PubMed PubMed Citation Articles by Ohnishi, K Articles by Iino, T

 Previous Article  |  Next Article 

J Bacteriol. 1987 April; 169(4): 1485-1488

Formation of flagella lacking outer rings by flaM, flaU, and flaY mutants of Escherichia coli.

ABSTRACT

Among flagellar mutants of Escherichia coli, flaM or flaU mutants form basal bodies lacking the outer P and L rings, whereas flaY mutants predominantly form basal bodies lacking the L ring. In these mutants, hooks and filaments are occasionally assembled onto these incomplete basal bodies. When the hook protein gene, flaFV, of Salmonella typhimurium was cloned on the multicopy plasmid pBR322 and introduced into these mutants, the efficiency with which cells assembled hooks and filaments onto the incomplete basal bodies increased significantly. Such cells formed characteristic dotted swarms on semisolid plates, indicating that cells carrying flagella without the outer rings are weakly motile because of poor function of their flagella, a low flagellar number per cell, or both of these defects. FlaV mutants also produced incomplete basal bodies lacking the outer rings, but assembly of hooks and filaments did not occur in these mutants even after introduction of the plasmid carrying flaFV of S. typhimurium. The failure in the case of flaV mutants was attributed to their inability to modify the rod tip to the structure competent for assembly of hook protein.

This article has been cited by other articles:

• Hizukuri, Y., Yakushi, T., Kawagishi, I., Homma, M. (2006). Role of the Intramolecular Disulfide Bond in FlgI, the Flagellar P-Ring Component of Escherichia coli.. J. Bacteriol. 188: 4190-4197 [Abstract] [Full Text]  
• Lee, V. T., Schneewind, O. (2001). Protein secretion and the pathogenesis of bacterial infections. Genes Dev. 15: 1725-1752 [Full Text]  
• Muramoto, K., Makishima, S., Aizawa, S.-I., Macnab, R. M. (1999). Effect of Hook Subunit Concentration on Assembly and Control of Length of the Flagellar Hook of Salmonella. J. Bacteriol. 181: 5808-5813 [Abstract] [Full Text]