This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Condemine, G Articles by Robert-Baudouy, J Search for Related Content PubMed PubMed Citation Articles by Condemine, G Articles by Robert-Baudouy, J

 Previous Article  |  Next Article 

J Bacteriol. 1987 May; 169(5): 1972-1978

2-keto-3-deoxygluconate transport system in Erwinia chrysanthemi.

ABSTRACT

In Erwinia chrysanthemi, the gene kdgT encodes a transport system responsible for the uptake of ketodeoxyuronates. We studied the biochemical properties of this transport system. The bacteria could grow on 2,5-diketo-3-deoxygluconate but not on 2-keto-3-deoxygluconate. The 2-keto-3-deoxygluconate entry reaction displayed saturation kinetics, with an apparent Km of 0.52 mM (at 30 degrees C and pH 7). 5-Keto-4-deoxyuronate and 2,5-diketo-3-deoxygluconate appeared to be competitive inhibitors, with Kis of 0.11 and 0.06 mM, respectively. The 2-keto-3-deoxygluconate permease could mediate the uptake of glucuronate with a low affinity. kdgT was cloned on an R-prime plasmid formed by in vivo complementation of a kdgT mutation of Escherichia coli. After being subcloned, it was mutagenized with a mini-Mu-lac transposable element able to form fusions with the lacZ gene. We introduced a kdgT-lac fusion into the E. chrysanthemi chromosome by marker exchange recombination and studied its regulation. kdgT product synthesis was not induced by external 2-keto-3-deoxygluconate in the wild-type strain but was induced by galacturonate and polygalacturonate. Two types of regulatory mutants able to grow on 2-keto-3-deoxygluconate as the sole carbon source were studied. Mutants of one group had a mutation in the operator region of kdgT; mutants of the other group had a mutation in kdgR, a regulatory gene controlling kdgT expression.

This article has been cited by other articles:

• Abbott, D. W., Boraston, A. B. (2008). Structural Biology of Pectin Degradation by Enterobacteriaceae. Microbiol. Mol. Biol. Rev. 72: 301-316 [Abstract] [Full Text]  
• Lin, J.-S., Shaw, G.-C. (2007). Regulation of the kduID operon of Bacillus subtilis by the KdgR repressor and the ccpA gene: identification of two KdgR-binding sites within the kdgR-kduI intergenic region. Microbiology 153: 701-710 [Abstract] [Full Text]  
• Rodionov, D. A., Gelfand, M. S., Hugouvieux-Cotte-Pattat, N. (2004). Comparative genomics of the KdgR regulon in Erwinia chrysanthemi 3937 and other gamma-proteobacteria. Microbiology 150: 3571-3590 [Abstract] [Full Text]  
• Laatu, M., Condemine, G. (2003). Rhamnogalacturonate Lyase RhiE Is Secreted by the Out System in Erwinia chrysanthemi. J. Bacteriol. 185: 1642-1649 [Abstract] [Full Text]  
• Condemine, G., Shevchik, V. E. (2000). Overproduction of the secretin OutD suppresses the secretion defect of an Erwinia chrysanthemi outB mutant. Microbiology 146: 639-647 [Abstract] [Full Text]