This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Coleman, J
Right arrow Articles by Raetz, C R
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Coleman, J
Right arrow Articles by Raetz, C R

 Previous Article  |  Next Article 

J Bacteriol. 1988 March; 170(3): 1268-1274

research-article

First committed step of lipid A biosynthesis in Escherichia coli: sequence of the lpxA gene.

J Coleman and C R Raetz

Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison 53706.

ABSTRACT

The min 4 region of the Escherichia coli genome contains genes (lpxA and lpxB) that encode proteins involved in lipid A biosynthesis. We have determined the sequence of 1,350 base pairs of DNA upstream of the lpxB gene. This fragment of DNA contains the complete coding sequence for the 28.0-kilodalton lpxA gene product and an upstream open reading frame capable of encoding a 17-kilodalton protein (ORF17). In addition there appears to be an additional open reading frame (ORF?) immediately upstream of ORF17. The initiation codon for lpxA is a GUG codon, and the start codon for ORF17 is apparently a UUG codon. The start and stop codons overlap between ORF? and ORF17, ORF17 and lpxA, and lpxA and lpxB. This overlap is suggestive of translational coupling and argues that the genes are cotranscribed. Crowell et al. (D.N. Crowell, W.S. Reznikoff, and C.R.H. Raetz, J. Bacteriol. 169:5727-5734, 1987) and Tomasiewicz and McHenry (H.G. Tomasiewicz and C.S. McHenry, J. Bacteriol. 169:5735-5744, 1987) have demonstrated that there are three similarly overlapping coding regions downstream of lpxB including dnaE, suggesting the existence of a complex operon of at least seven genes: 5'-ORF?-ORF17-lpxA-lpxB-ORF23-dnaE-ORF37-3 '.

J Bacteriol. 1988 March; 170(3): 1268-1274




This article has been cited by other articles:

  • Geurtsen, J., Angevaare, E., Janssen, M., Hamstra, H.-J., Hove, J. t., de Haan, A., Kuipers, B., Tommassen, J., van der Ley, P. (2007). A Novel Secondary Acyl Chain in the Lipopolysaccharide of Bordetella pertussis Required for Efficient Infection of Human Macrophages. J. Biol. Chem. 282: 37875-37884 [Abstract] [Full Text]  
  • Williams, A. H., Raetz, C. R. H. (2007). Inaugural Article: Structural basis for the acyl chain selectivity and mechanism of UDP-N-acetylglucosamine acyltransferase. Proc. Natl. Acad. Sci. USA 104: 13543-13550 [Abstract] [Full Text]  
  • Trent, M. S., Stead, C. M., Tran, A. X., Hankins, J. V. (2006). Invited review: Diversity of endotoxin and its impact on pathogenesis. Innate Immunity 12: 205-223 [Abstract]  
  • Sweet, C. R., Ribeiro, A. A., Raetz, C. R. H. (2004). Oxidation and Transamination of the 3''-Position of UDP-N-Acetylglucosamine by Enzymes from Acidithiobacillus ferrooxidans: ROLE IN THE FORMATION OF LIPID A MOLECULES WITH FOUR AMIDE-LINKED ACYL CHAINS. J. Biol. Chem. 279: 25400-25410 [Abstract] [Full Text]  
  • Clements, J. M., Coignard, F., Johnson, I., Chandler, S., Palan, S., Waller, A., Wijkmans, J., Hunter, M. G. (2002). Antibacterial Activities and Characterization of Novel Inhibitors of LpxC. Antimicrob. Agents Chemother. 46: 1793-1799 [Abstract] [Full Text]  
  • Sweet, C. R., Preston, A., Toland, E., Ramirez, S. M., Cotter, R. J., Maskell, D. J., Raetz, C. R. H. (2002). Relaxed Acyl Chain Specificity of Bordetella UDP-N-acetylglucosamine Acyltransferases. J. Biol. Chem. 277: 18281-18290 [Abstract] [Full Text]  
  • Preston, A., Maskell, D. (2001). Invited review: The molecular genetics and role in infection of lipopolysaccharide biosynthesis in the Bordetellae. Innate Immunity 7: 251-261  
  • Steeghs, L., Kuipers, B., Hamstra, H. J., Kersten, G., van Alphen, L., van der Ley, P. (1999). Immunogenicity of Outer Membrane Proteins in a Lipopolysaccharide-Deficient Mutant of Neisseria meningitidis: Influence of Adjuvants on the Immune Response. Infect. Immun. 67: 4988-4993 [Abstract] [Full Text]  
  • Wyckoff, T. J. O., Raetz, C. R. H. (1999). The Active Site of Escherichia coli UDP-N-acetylglucosamine Acyltransferase. CHEMICAL MODIFICATION AND SITE-DIRECTED MUTAGENESIS. J. Biol. Chem. 274: 27047-27055 [Abstract] [Full Text]  
  • Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]  
  • Stanley, P., Koronakis, V., Hughes, C. (1998). Acylation of Escherichia coli Hemolysin: A Unique Protein Lipidation Mechanism Underlying Toxin Function. Microbiol. Mol. Biol. Rev. 62: 309-333 [Abstract] [Full Text]  
  • Dotson, G. D., Kaltashov, I. A., Cotter, R. J., Raetz, C. R. H. (1998). Expression Cloning of a Pseudomonas Gene Encoding a Hydroxydecanoyl-Acyl Carrier Protein-Dependent UDP-GlcNAc Acyltransferase. J. Bacteriol. 180: 330-337 [Abstract] [Full Text]  
  • Odegaard, T. J., Kaltashov, I. A., Cotter, R. J., Steeghs, L., van der Ley, P., Khan, S., Maskell, D. J., Raetz, C. R. H. (1997). Shortened Hydroxyacyl Chains on Lipid A of Escherichia coli Cells Expressing a Foreign UDP-N-Acetylglucosamine O-Acyltransferase. J. Biol. Chem. 272: 19688-19696 [Abstract] [Full Text]  
  • Brozek, K. A., Carlson, R. W., Raetz, C. R.H. (1996). A Special Acyl Carrier Protein for Transferring Long Hydroxylated Fatty Acids to Lipid A in Rhizobium. J. Biol. Chem. 271: 32126-32136 [Abstract] [Full Text]  
  • Sorensen, P. G., Lutkenhaus, J., Young, K., Eveland, S. S., Anderson, M. S., Raetz, C. R.H. (1996). Regulation of UDP-3-O-[R-3-hydroxymyristoyl]-N-acetylglucosamine Deacetylase in Escherichia coli. THE SECOND ENZYMATIC STEP OF LIPID A BIOSYNTHESIS. J. Biol. Chem. 271: 25898-25905 [Abstract] [Full Text]  
  • Clementz, T., Bednarski, J. J., Raetz, C. R. H. (1996). Function of the htrB High Temperature Requirement Gene of Escherichia coli in the Acylation of Lipid A. J. Biol. Chem. 271: 12095-12102 [Abstract] [Full Text]  
  • Raetz, C. R. H., Roderick, S. L. (1995). A Left-Handed Parallel beta Helix in the Structure of UDP-N-Acetylglucosamine Acyltransferase. Science 270: 997-1000 [Abstract]  
  • Shih, S. J., Nelson, D. L. (1992). Proteolytic processing of secretory proteins in Paramecium: immunological and biochemical characterization of the precursors of trichocyst matrix proteins. J. Cell Sci. 103: 349-361 [Abstract]  
  • Sweet, C. R., Lin, S., Cotter, R. J., Raetz, C. R. H. (2001). A Chlamydia trachomatis UDP-N-Acetylglucosamine Acyltransferase Selective for Myristoyl-Acyl Carrier Protein. EXPRESSION IN ESCHERICHIA COLI AND FORMATION OF HYBRID LIPID A SPECIES. J. Biol. Chem. 276: 19565-19574 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»