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J Bacteriol. 1988 June; 170(6): 2879-2883

research-article

Nucleotide sequence and organization of copper resistance genes from Pseudomonas syringae pv. tomato.

Department of Plant Pathology, University of California, Riverside 92521.

ABSTRACT

The nucleotide sequence of a 4.5-kilobase copper resistance determinant from Pseudomonas syringae pv. tomato revealed four open reading frames (ORFs) in the same orientation. Deletion and site-specific mutational analyses indicated that the first two ORFs were essential for copper resistance; the last two ORFs were required for full resistance, but low-level resistance could be conferred in their absence. Five highly conserved, direct 24-base repeats were found near the beginning of the second ORF, and a similar, but less conserved, repeated region was found in the middle of the first ORF.

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