This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Byrne, C R Articles by Kredich, N M Search for Related Content PubMed PubMed Citation Articles by Byrne, C R Articles by Kredich, N M

research-article

DNA sequences of the cysK regions of Salmonella typhimurium and Escherichia coli and linkage of the cysK regions to ptsH.

Division of Animal Production, Commonwealth Scientific and Industrial Research Organisation, Blacktown, New South Wales, Australia.

ABSTRACT

Nucleotide sequences of the cysK regions of Salmonella typhimurium and Escherichia coli have been determined. A total of 3,812 and 2,595 nucleotides were sequenced from S. typhimurium and E. coli, respectively. Open reading frames of 323 codons were found in both species and were identified as those of cysK by comparison of deduced amino acid sequences with amino- and carboxyl-terminal amino acid analyses of the S. typhimurium cysK gene product O-acetylserine (thiol)-lyase A. The two cysK DNA sequences were 85% identical, and the deduced amino acid sequences were 96% identical. The major transcription initiation sites for cysK were found to be virtually identical in the two organisms, by using primer extension and S1 nuclease protection techniques. The -35 region corresponding to the major transcription start site was TTCCCC in S. typhimurium and TTCCGC in E. coli. The deviation of these sequences from the consensus sequence TTGACA may reflect the fact that cysK is subject to positive control and requires the cysB regulatory protein for expression. Sequences downstream of cysK were found to include ptsH and a portion of ptsI, thus establishing the exact relationship of cysK with these two genes. A 290-codon open reading frame, which may represent the cysZ gene, was identified upstream of cysK.

This article has been cited by other articles:

• Du, Y., Arvidson, C. G. (2006). RpoH Mediates the Expression of Some, but Not All, Genes Induced in Neisseria gonorrhoeae Adherent to Epithelial Cells.. Infect. Immun. 74: 2767-2776 [Abstract] [Full Text]  
• Yamamoto, K., Ishihama, A. (2005). Transcriptional Response of Escherichia coli to External Zinc. J. Bacteriol. 187: 6333-6340 [Abstract] [Full Text]  
• Oliveriusova, J., Kery, V., Maclean, K. N., Kraus, J. P. (2002). Deletion Mutagenesis of Human Cystathionine beta -Synthase. IMPACT ON ACTIVITY, OLIGOMERIC STATUS, AND S-ADENOSYLMETHIONINE REGULATION. J. Biol. Chem. 277: 48386-48394 [Abstract] [Full Text]  
• Phadtare, S., Kato, I., Inouye, M. (2002). DNA Microarray Analysis of the Expression Profile of Escherichia coli in Response to Treatment with 4,5-Dihydroxy-2-Cyclopenten-1-One. J. Bacteriol. 184: 6725-6729 [Abstract] [Full Text]  
• Fernández, M., van Doesburg, W., Rutten, G. A. M., Marugg, J. D., Alting, A. C., van Kranenburg, R., Kuipers, O. P. (2000). Molecular and Functional Analyses of the metC Gene of Lactococcus lactis, Encoding Cystathionine beta -Lyase. Appl. Environ. Microbiol. 66: 42-48 [Abstract] [Full Text]  
• Borup, B., Ferry, J. G. (2000). O-Acetylserine Sulfhydrylase from Methanosarcina thermophila. J. Bacteriol. 182: 45-50 [Abstract] [Full Text]  
• Schnackerz, K. D., Tai, C.-H., Potsch, R. K. W., Cook, P. F. (1999). Substitution of Pyridoxal 5'-Phosphate in D-Serine Dehydratase from Escherichia coli by Cofactor Analogues Provides Information on Cofactor Binding and Catalysis. J. Biol. Chem. 274: 36935-36943 [Abstract] [Full Text]  
• Rather, P. N., Ding, X., Baca-DeLancey, R. R., Siddiqui, S. (1999). Providencia stuartii Genes Activated by Cell-to-Cell Signaling and Identification of a Gene Required for Production or Activity of an Extracellular Factor. J. Bacteriol. 181: 7185-7191 [Abstract] [Full Text]  
• Baca-DeLancey, R. R., South, M. M. T., Ding, X., Rather, P. N. (1999). Escherichia coli genes regulated by cell-to-cell signaling. Proc. Natl. Acad. Sci. USA 96: 4610-4614 [Abstract] [Full Text]  
• Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]  
• Flint, D. H., Tuminello, J. F., Miller, T. J. (1996). Studies on the Synthesis of the Fe-S Cluster of Dihydroxy-acid Dehydratase in Escherichia coli Crude Extract. ISOLATION OF O-ACETYLSERINE SULFHYDRYLASES A AND B AND beta -CYSTATHIONASE BASED ON THEIR ABILITY TO MOBILIZE SULFUR FROM CYSTEINE AND TO PARTICIPATE IN Fe-S CLUSTER SYNTHESIS. J. Biol. Chem. 271: 16053-16067 [Abstract] [Full Text]  
• Powell, B. S., Court, D. L., Inada, T., Nakamura, Y., Michotey, V., Cui, X., Reizer, A., Saier, M. H. Jr., Reizer, J. (1995). Novel Proteins of the Phosphotransferase System Encoded within the rpoN Operon of Escherichia coli. J. Biol. Chem. 270: 4822-4839 [Abstract] [Full Text]  
• Ryu, S., Ramseier, T. M., Michotey, V., Saier, M. H. Jr., Garges, S. (1995). Effect of the FruR Regulator on Transcription of the pts Operon in Escherichia coli. J. Biol. Chem. 270: 2489-2496 [Abstract] [Full Text]