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J Bacteriol. 1988 August; 170(8): 3443-3447

research-article

A deficiency in cyclic AMP results in pH-sensitive growth of Escherichia coli K-12.

D Ahmad and E B Newman

Department of Biological Sciences, Concordia University, Montreal, Quebec, Canada.

ABSTRACT

Mutants of Escherichia coli K-12 deficient in adenyl cyclase (cya) and catabolite activator protein (crp) have been shown to grow more slowly than their parent strains in glucose-minimal medium. Their growth rate decreased markedly with increasing pH between 6 and 7.8. We have shown that this pH sensitivity is a direct consequence of the cya mutation, because a mutation to pH resistance also restored ability to ferment a variety of sugars. The proton motive force-dependent uptake of proline and glutamate was also reduced and sensitive to pH in the cya mutant. The membrane-bound ATPase activity was normal. The rate of oxygen uptake by cells, although reduced, was pH insensitive. We suggest several explanations for this phenotype, including a possible defect in energy transduction.

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J Bacteriol. 1988 August; 170(8): 3443-3447

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