This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Thomashow, L S Articles by Weller, D M Search for Related Content PubMed PubMed Citation Articles by Thomashow, L S Articles by Weller, D M

research-article

Role of a phenazine antibiotic from Pseudomonas fluorescens in biological control of Gaeumannomyces graminis var. tritici.

U.S. Department of Agriculture, Root Disease and Biological Control Research Unit, Washington State University, Pullman 99164-6430.

ABSTRACT

Pseudomonas fluorescens 2-79 (NRRL B-15132) and its rifampin-resistant derivative 2-79RN10 are suppressive to take-all, a major root disease of wheat caused by Gaeumannomyces graminis var. tritici. Strain 2-79 produces the antibiotic phenazine-1-carboxylate, which is active in vitro against G. graminis var. tritici and other fungal root pathogens. Mutants defective in phenazine synthesis (Phz-) were generated by Tn5 insertion and then compared with the parental strain to determine the importance of the antibiotic in take-all suppression on wheat roots. Six independent, prototrophic Phz- mutants were noninhibitory to G. graminis var. tritici in vitro and provided significantly less control of take-all than strain 2-79 on wheat seedlings. Antibiotic synthesis, fungal inhibition in vitro, and suppression of take-all on wheat were coordinately restored in two mutants complemented with cloned DNA from a 2-79 genomic library. These mutants contained Tn5 insertions in adjacent EcoRI fragments in the 2-79 genome, and the restriction maps of the region flanking the insertions and the complementary DNA were colinear. These results indicate that sequences required for phenazine production were present in the cloned DNA and support the importance of the phenazine antibiotic in disease suppression in the rhizosphere.

This article has been cited by other articles:

• Maddula, V. S. R. K., Pierson, E. A., Pierson, L. S. III (2008). Altering the Ratio of Phenazines in Pseudomonas chlororaphis (aureofaciens) Strain 30-84: Effects on Biofilm Formation and Pathogen Inhibition. J. Bacteriol. 190: 2759-2766 [Abstract] [Full Text]  
• Khan, S. R., Herman, J., Krank, J., Serkova, N. J., Churchill, M. E. A., Suga, H., Farrand, S. K. (2007). N-(3-Hydroxyhexanoyl)-L-Homoserine Lactone Is the Biologically Relevant Quormone That Regulates the phz Operon of Pseudomonas chlororaphis Strain 30-84. Appl. Environ. Microbiol. 73: 7443-7455 [Abstract] [Full Text]  
• Krishnan, H. B., Kang, B. R., Hari Krishnan, A., Kim, K. Y., Kim, Y. C. (2007). Rhizobium etli USDA9032 Engineered To Produce a Phenazine Antibiotic Inhibits the Growth of Fungal Pathogens but Is Impaired in Symbiotic Performance. Appl. Environ. Microbiol. 73: 327-330 [Abstract] [Full Text]  
• Selim, S., Negrel, J., Govaerts, C., Gianinazzi, S., van Tuinen, D. (2005). Isolation and Partial Characterization of Antagonistic Peptides Produced by Paenibacillus sp. Strain B2 Isolated from the Sorghum Mycorrhizosphere. Appl. Environ. Microbiol. 71: 6501-6507 [Abstract] [Full Text]  
• Khan, S. R., Mavrodi, D. V., Jog, G. J., Suga, H., Thomashow, L. S., Farrand, S. K. (2005). Activation of the phz Operon of Pseudomonas fluorescens 2-79 Requires the LuxR Homolog PhzR, N-(3-OH-Hexanoyl)-L-Homoserine Lactone Produced by the LuxI Homolog PhzI, and a cis-Acting phz Box. J. Bacteriol. 187: 6517-6527 [Abstract] [Full Text]  
• Barea, J.-M., Pozo, M. J., Azcon, R., Azcon-Aguilar, C. (2005). Microbial co-operation in the rhizosphere. J Exp Bot 56: 1761-1778 [Abstract] [Full Text]  
• Wang, Y.-J., Leadbetter, J. R. (2005). Rapid Acyl-Homoserine Lactone Quorum Signal Biodegradation in Diverse Soils. Appl. Environ. Microbiol. 71: 1291-1299 [Abstract] [Full Text]  
• Silby, M. W., Giddens, S. R., Mahanty, H. K. (2005). Mutation of a LysR-Type Regulator of Antifungal Activity Results in a Growth Advantage in Stationary Phase Phenotype in Pseudomonas aureofaciens PA147-2. Appl. Environ. Microbiol. 71: 569-573 [Abstract] [Full Text]  
• Blankenfeldt, W., Kuzin, A. P., Skarina, T., Korniyenko, Y., Tong, L., Bayer, P., Janning, P., Thomashow, L. S., Mavrodi, D. V. (2004). Structure and function of the phenazine biosynthetic protein PhzF from Pseudomonas fluorescens. Proc. Natl. Acad. Sci. USA 101: 16431-16436 [Abstract] [Full Text]  
• Ovadis, M., Liu, X., Gavriel, S., Ismailov, Z., Chet, I., Chernin, L. (2004). The Global Regulator Genes from Biocontrol Strain Serratia plymuthica IC1270: Cloning, Sequencing, and Functional Studies. J. Bacteriol. 186: 4986-4993 [Abstract] [Full Text]  
• Ownley, B. H., Duffy, B. K., Weller, D. M. (2003). Identification and Manipulation of Soil Properties To Improve the Biological Control Performance of Phenazine-Producing Pseudomonas fluorescens. Appl. Environ. Microbiol. 69: 3333-3343 [Abstract] [Full Text]  
• Cheng, Y., McNally, D. J., Labbe, C., Voyer, N., Belzile, F., Belanger, R. R. (2003). Insertional Mutagenesis of a Fungal Biocontrol Agent Led to Discovery of a Rare Cellobiose Lipid with Antifungal Activity. Appl. Environ. Microbiol. 69: 2595-2602 [Abstract] [Full Text]  
• Lee, J. Y., Moon, S. S., Hwang, B. K. (2003). Isolation and Antifungal and Antioomycete Activities of Aerugine Produced by Pseudomonas fluorescens Strain MM-B16. Appl. Environ. Microbiol. 69: 2023-2031 [Abstract] [Full Text]  
• Dunn, A. K., Klimowicz, A. K., Handelsman, J. (2003). Use of a Promoter Trap To Identify Bacillus cereus Genes Regulated by Tomato Seed Exudate and a Rhizosphere Resident, Pseudomonas aureofaciens. Appl. Environ. Microbiol. 69: 1197-1205 [Abstract] [Full Text]  
• Andersen, J. B., Koch, B., Nielsen, T. H., Sorensen, D., Hansen, M., Nybroe, O., Christophersen, C., Sorensen, J., Molin, S., Givskov, M. (2003). Surface motility in Pseudomonas sp. DSS73 is required for efficient biological containment of the root-pathogenic microfungi Rhizoctonia solani and Pythium ultimum. Microbiology 149: 37-46 [Abstract] [Full Text]  
• Glandorf, D. C. M., Verheggen, P., Jansen, T., Jorritsma, J.-W., Smit, E., Leeflang, P., Wernars, K., Thomashow, L. S., Laureijs, E., Thomas-Oates, J. E., Bakker, P. A. H. M., van Loon, L. C. (2001). Effect of Genetically Modified Pseudomonas putida WCS358r on the Fungal Rhizosphere Microflora of Field-Grown Wheat. Appl. Environ. Microbiol. 67: 3371-3378 [Abstract] [Full Text]  
• Sigler, W. V., Nakatsu, C. H., Reicher, Z. J., Turco, R. F. (2001). Fate of the Biological Control Agent Pseudomonas aureofaciens TX-1 after Application to Turfgrass. Appl. Environ. Microbiol. 67: 3542-3548 [Abstract] [Full Text]  
• Raaijmakers, J. M., Weller, D. M. (2001). Exploiting Genotypic Diversity of 2,4-Diacetylphloroglucinol-Producing Pseudomonas spp.: Characterization of Superior Root-Colonizing P. fluorescens Strain Q8r1-96. Appl. Environ. Microbiol. 67: 2545-2554 [Abstract] [Full Text]  
• Delaney, S. M., Mavrodi, D. V., Bonsall, R. F., Thomashow, L. S. (2001). phzO, a Gene for Biosynthesis of 2-Hydroxylated Phenazine Compounds in Pseudomonas aureofaciens 30-84. J. Bacteriol. 183: 318-327 [Abstract] [Full Text]  
• Picard, C., Di Cello, F., Ventura, M., Fani, R., Guckert, A. (2000). Frequency and Biodiversity of 2,4-Diacetylphloroglucinol-Producing Bacteria Isolated from the Maize Rhizosphere at Different Stages of Plant Growth. Appl. Environ. Microbiol. 66: 948-955 [Abstract] [Full Text]  
• Nakayama, T., Homma, Y., Hashidoko, Y., Mizutani, J., Tahara, S. (1999). Possible Role of Xanthobaccins Produced by Stenotrophomonas sp. Strain SB-K88 in Suppression of Sugar Beet Damping-Off Disease. Appl. Environ. Microbiol. 65: 4334-4339 [Abstract] [Full Text]  
• Nielsen, M. N., Sørensen, J., Fels, J., Pedersen, H. C. (1998). Secondary Metabolite- and Endochitinase-Dependent Antagonism toward Plant-Pathogenic Microfungi of Pseudomonas fluorescens Isolates from Sugar Beet Rhizosphere. Appl. Environ. Microbiol. 64: 3563-3569 [Abstract] [Full Text]  
• Kang, Y., Carlson, R., Tharpe, W., Schell, M. A. (1998). Characterization of Genes Involved in Biosynthesis of a Novel Antibiotic from Burkholderia cepacia BC11 and Their Role in Biological Control of Rhizoctonia solani. Appl. Environ. Microbiol. 64: 3939-3947 [Abstract] [Full Text]  
• Mavrodi, D. V., Ksenzenko, V. N., Bonsall, R. F., Cook, R. J., Boronin, A. M., Thomashow, L. S. (1998). A Seven-Gene Locus for Synthesis of Phenazine-1-Carboxylic Acid by Pseudomonas fluorescens 2-79. J. Bacteriol. 180: 2541-2548 [Abstract] [Full Text]  
• Lindow, S., Panopoulos, N., McFarland, B. (1989). Genetic engineering of bacteria from managed and natural habitats. Science 244: 1300-1307 [Abstract]