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The promoter for a sporulation gene in the spoIVC locus of Bacillus subtilis and its use in studies of temporal and spatial control of gene expression.

Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138.

ABSTRACT

We have identified the transcription start site and regulatory region governing the expression of a sporulation gene in the spoIVC locus of Bacillus subtilis. Efficient expression and developmental regulation of this gene was controlled from a promoter region that extended no more than 110 base pairs upstream and no more than 4 base pairs downstream from the start site of transcription, on which basis we infer that spoIVC is regulated at the level of transcription initiation. Using a transcriptional fusion of the spoIVC gene to the lacZ gene of Escherichia coli, we found that spoIVC expression was turned on at the third to fourth hour of sporulation (at about the developmental stage [IV] that its products are required in spore formation) and that this transcription was largely restricted to the mother cell chamber of the sporangium. Mutations in many different spo genes (causing blocks at stages 0 to V) were found to influence (negatively and positively) the level of spoIVC expression. Our results distinguish the mode of spoIVC regulation from that of previously studied sporulation genes and indicate that it is representative of a new regulon of mother cell-specific gene expression.

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• Wade, K. H., Schyns, G., Opdyke, J. A., Moran, C. P. Jr. (1999). A Region of sigma K Involved in Promoter Activation by GerE in Bacillus subtilis. J. Bacteriol. 181: 4365-4373 [Abstract] [Full Text]  
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• Driks, A. (1999). Bacillus subtilis Spore Coat. Microbiol. Mol. Biol. Rev. 63: 1-20 [Abstract] [Full Text]  
• Takamatsu, H., Chikahiro, Y., Kodama, T., Koide, H., Kozuka, S., Tochikubo, K., Watabe, K. (1998). A Spore Coat Protein, CotS, of Bacillus subtilis Is Synthesized under the Regulation of sigma K and GerE during Development and Is Located in the Inner Coat Layer of Spores. J. Bacteriol. 180: 2968-2974 [Abstract] [Full Text]  
• Sato, T., Kobayashi, Y. (1998). The ars Operon in the skin Element of Bacillus subtilis Confers Resistance to Arsenate and Arsenite. J. Bacteriol. 180: 1655-1661 [Abstract] [Full Text]  
• Driks, A, Roels, S, Beall, B, Moran, C P, Losick, R (1994). Subcellular localization of proteins involved in the assembly of the spore coat of Bacillus subtilis.. Genes Dev. 8: 234-244 [Abstract]  
• Cutting, S, Driks, A, Schmidt, R, Kunkel, B, Losick, R (1991). Forespore-specific transcription of a gene in the signal transduction pathway that governs Pro-sigma K processing in Bacillus subtilis.. Genes Dev. 5: 456-466 [Abstract]  
• Kunkel, B, Losick, R, Stragier, P (1990). The Bacillus subtilis gene for the development transcription factor sigma K is generated by excision of a dispensable DNA element containing a sporulation recombinase gene.. Genes Dev. 4: 525-535 [Abstract]  
• Kunkel, B, Kroos, L, Poth, H, Youngman, P, Losick, R (1989). Temporal and spatial control of the mother-cell regulatory gene spoIIID of Bacillus subtilis.. Genes Dev. 3: 1735-1744 [Abstract]  
• Sun, D X, Stragier, P, Setlow, P (1989). Identification of a new sigma-factor involved in compartmentalized gene expression during sporulation of Bacillus subtilis.. Genes Dev. 3: 141-149 [Abstract]  
• Karmazyn-Campelli, C, Bonamy, C, Savelli, B, Stragier, P (1989). Tandem genes encoding sigma-factors for consecutive steps of development in Bacillus subtilis.. Genes Dev. 3: 150-157 [Abstract]  
• Stragier, P, Kunkel, B, Kroos, L, Losick, R (1989). Chromosomal rearrangement generating a composite gene for a developmental transcription factor. Science 243: 507-512 [Abstract]  
• Kroos, L, Kunkel, B, Losick, R (1989). Switch protein alters specificity of RNA polymerase containing a compartment-specific sigma factor. Science 243: 526-529 [Abstract]  
• Li, Z., Piggot, P. J. (2001). Development of a two-part transcription probe to determine the completeness of temporal and spatial compartmentalization of gene expression during bacterial development. Proc. Natl. Acad. Sci. USA 98: 12538-12543 [Abstract] [Full Text]