This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mizunoe, Y Articles by Amako, K Search for Related Content PubMed PubMed Citation Articles by Mizunoe, Y Articles by Amako, K

research-article

Cloning and sequence of the gene encoding the major structural component of mannose-resistant fimbriae of Serratia marcescens.

Department of Bacteriology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.

ABSTRACT

Serratia marcescens US46, a human urinary tract isolate, exhibits mannose-resistant hemagglutination and agglutinates yeast cells, thereby indicating that it has two types of adhesins. We constructed a cosmid library for the DNA of this organism and isolated DNA clones carrying genes for mannose-sensitive (MS) and mannose-resistant (MR) fimbriae. On introduction of the cloned genes into Escherichia coli K-12, MS and MR fimbriae were formed. These fimbriae were functionally and morphologically indistinguishable from those of S. marcescens. Subcloning of these gene clusters revealed that the genes encoding MS fimbriae reside on a 9-kilobase (kb) DNA fragment, while those encoding MR fimbriae are present on a 12-kb fragment. Transposon insertion and maxicell analyses revealed that formation of MR fimbriae is controlled by several genes which reside on the 9-kb fragment. The nucleotide sequence of smfA, the gene encoding the major structural component of MR fimbriae, revealed that this gene encodes a 174-amino-acid polypeptide with a typical procaryotic signal peptide. The primary structure of the smfA product showed significant homology with the primary structure of the E. coli fimbrial subunit.

This article has been cited by other articles:

• Shanks, R. M. Q., Stella, N. A., Kalivoda, E. J., Doe, M. R., O'Dee, D. M., Lathrop, K. L., Guo, F. L., Nau, G. J. (2007). A Serratia marcescens OxyR Homolog Mediates Surface Attachment and Biofilm Formation. J. Bacteriol. 189: 7262-7272 [Abstract] [Full Text]  
• Johnson, J. R., Stell, A. L., Scheutz, F., O'Bryan, T. T., Russo, T. A., Carlino, U. B., Fasching, C., Kavle, J., Van Dijk, L., Gaastra, W. (2000). Analysis of the F Antigen-Specific papA Alleles of Extraintestinal Pathogenic Escherichia coli Using a Novel Multiplex PCR-Based Assay. Infect. Immun. 68: 1587-1599 [Abstract] [Full Text]  
• Morrow, B. J., Graham, J. E., Curtiss, R. III (1999). Genomic Subtractive Hybridization and Selective Capture of Transcribed Sequences Identify a Novel Salmonella typhimurium Fimbrial Operon and Putative Transcriptional Regulator That Are Absent from the Salmonella typhi Genome. Infect. Immun. 67: 5106-5116 [Abstract] [Full Text]