This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Golden, S S Articles by Cho, D S Search for Related Content PubMed PubMed Citation Articles by Golden, S S Articles by Cho, D S

 Previous Article  |  Next Article 

J Bacteriol. 1989 January; 171(1): 24-29

research-article

Genetic relationship of two highly studied Synechococcus strains designated Anacystis nidulans.

Department of Biology, Texas A&M University, College Station 77843-3258.

ABSTRACT

The cyanobacteria Synechococcus sp. strain PCC 7942 and Synechococcus sp. strain PCC 6301 are very closely related and both have been designated by the binomial Anacystis nidulans. The only established difference between the two strains is the superior transformation properties of strain PCC 7942. Significant homology between the rRNA genes of these strains was demonstrated by the ability of an rRNA operon from strain PCC 6301, interrupted by a spectinomycin and streptomycin resistance marker, to transform strain PCC 7942 by recombining with and replacing an endogenous rRNA operon. Restriction fragment length polymorphism data indicated that the chromosomes of the two strains were conserved around the three psbA loci, the two rRNA operons, and the psbDI locus. However, multiple polymorphisms were detected downstream of the psbDII locus, identifying a DNA rearrangement such as an inversion, insertion, or deletion within the chromosome. Analysis of genome structure by pulsed-field gel electrophoresis of large NotI restriction fragments showed only two bands that were visibly shifted between the chromosomes of the two strains. These data support their very close genetic relationship and the feasibility of studying genes derived from strain PCC 6301 in the highly transformable PCC 7942 strain.

This article has been cited by other articles:

• Ashby, M. K., Houmard, J. (2006). Cyanobacterial Two-Component Proteins: Structure, Diversity, Distribution, and Evolution. Microbiol. Mol. Biol. Rev. 70: 472-509 [Abstract] [Full Text]  
• Monshupanee, T., Fa-aroonsawat, S., Chungjatupornchai, W. (2006). A cyanobacterial strain with all chromosomal rRNA operons inactivated: a single nucleotide mutation of 23S rRNA confers temperature-sensitive phenotypes.. Microbiology 152: 1417-1425 [Abstract] [Full Text]  
• Matsuoka, M., Takahama, K., Ogawa, T. (2001). Gene replacement in cyanobacteria mediated by a dominant streptomycin-sensitive rps12 gene that allows selection of mutants free from drug resistance markers. Microbiology 147: 2077-2087 [Abstract] [Full Text]  
• Miller, A. G., Hunter, K. J., O'Leary, S. J.B., Hart, L. J. (2000). The Photoreduction of H2O2 by Synechococcus sp. PCC 7942 and UTEX 625. Plant Physiol. 123: 625-636 [Abstract] [Full Text]  
• Sakamoto, T., Bryant, D. A. (1999). Nitrate Transport and Not Photoinhibition Limits Growth of the Freshwater Cyanobacterium Synechococcus Species PCC 6301 at Low Temperature. Plant Physiol. 119: 785-794 [Abstract] [Full Text]  
• Knowles, V. L., Smith, C. S., Smith, C. R., Plaxton, W. C. (2001). Structural and Regulatory Properties of Pyruvate Kinase from the Cyanobacterium Synechococcus PCC 6301. J. Biol. Chem. 276: 20966-20972 [Abstract] [Full Text]