This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fisher, R F Articles by Long, S R Search for Related Content PubMed PubMed Citation Articles by Fisher, R F Articles by Long, S R

research-article

DNA footprint analysis of the transcriptional activator proteins NodD1 and NodD3 on inducible nod gene promoters.

Department of Biological Sciences, Stanford University, California 94305-5020.

ABSTRACT

The Rhizobium meliloti nodD1 and nodD3 gene products (NodD1 and NodD3) are members of the lysR-nodD gene regulator family. They are functionally distinct in that NodD1 transcriptionally activates other nod genes in the presence of a flavonoid inducer such as luteolin, while NodD3 is capable of activating nod gene expression at high levels in the absence of inducer. NodD1 and NodD3 are DNA-binding proteins which interact with DNA sequences situated upstream of the transcription initiation sites of at least three sets of inducible nod genes. We report the footprinting of NodD1- and NodD3-DNA complexes with both DNase I and the 1,10-phenanthroline-copper ion reagent. NodD1 and NodD3 both interacted with the nodABC, nodFE, and nodH promoters and protected from cleavage an extensive piece of DNA, including the nod box, from approximately -20 to -75 from the transcription start site for each of the three promoters. The constitutively activating protein NodD3 displayed an additional hypersensitive cleavage site in its footprint compared with NodD1.

This article has been cited by other articles:

• MacLean, A. M., Anstey, M. I., Finan, T. M. (2008). Binding Site Determinants for the LysR-Type Transcriptional Regulator PcaQ in the Legume Endosymbiont Sinorhizobium meliloti. J. Bacteriol. 190: 1237-1246 [Abstract] [Full Text]  
• Peck, M. C., Fisher, R. F., Long, S. R. (2006). Diverse Flavonoids Stimulate NodD1 Binding to nod Gene Promoters in Sinorhizobium meliloti.. J. Bacteriol. 188: 5417-5427 [Abstract] [Full Text]  
• Brencic, A., Winans, S. C. (2005). Detection of and Response to Signals Involved in Host-Microbe Interactions by Plant-Associated Bacteria. Microbiol. Mol. Biol. Rev. 69: 155-194 [Abstract] [Full Text]  
• Feng, J., Li, Q., Hu, H.-L., Chen, X.-C., Hong, G.-F. (2003). Inactivation of the nod box distal half-site allows tetrameric NodD to activate nodA transcription in an inducer-independent manner. Nucleic Acids Res 31: 3143-3156 [Abstract] [Full Text]  
• Yeh, K.-C., Peck, M. C., Long, S. R. (2002). Luteolin and GroESL Modulate In Vitro Activity of NodD. J. Bacteriol. 184: 525-530 [Abstract] [Full Text]  
• Loh, J., Stacey, G. (2002). Nodulation Gene Regulation in Bradyrhizobium japonicum: a Unique Integration of Global Regulatory Circuits. Appl. Environ. Microbiol. 69: 10-17 [Full Text]  
• Hu, H., Liu, S., Yang, Y., Chang, W., Hong, G. (2000). In Rhizobium leguminosarum, NodD represses its own transcription by competing with RNA polymerase for binding sites. Nucleic Acids Res 28: 2784-2793 [Abstract] [Full Text]  
• Strausak, D., Solioz, M. (1997). CopY Is a Copper-inducible Repressor of the Enterococcus hirae Copper ATPases. J. Biol. Chem. 272: 8932-8936 [Abstract] [Full Text]  
• Ogawa, J, Long, S R (1995). The Rhizobium meliloti groELc locus is required for regulation of early nod genes by the transcription activator NodD.. Genes Dev. 9: 714-729 [Abstract]  
• Storz, G, Tartaglia, L., Ames, B. (1990). Transcriptional regulator of oxidative stress-inducible genes: direct activation by oxidation. Science 248: 189-194 [Abstract]