This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dong, X N Articles by Rownd, R H Search for Related Content PubMed PubMed Citation Articles by Dong, X N Articles by Rownd, R H

research-article

DNA bending near the replication origin of IncFII plasmid NR1.

Department of Molecular Biology, Medical School, Northwestern University, Chicago, Illinois 60611.

ABSTRACT

The DNA replication origin of plasmid NR1 is located approximately 190 base pairs downstream from the 3' end of the repA1 gene, which encodes the essential initiation protein for replication of the plasmid. Restriction endonuclease fragments that contain the NR1 replication origin and its flanking sequences at circularly permuted positions were obtained by digesting oligomers of ori-containing DNA fragments with sets of enzymes that each cut only once in every ori fragment. Polyacrylamide gel electrophoresis of these permuted restriction fragments showed anomalous mobilities, indicating the presence of a DNA bending locus. Through analysis of the relative mobility plots of these permuted fragments, we found one or two possible DNA bending sites located in the intervening region between the repA1 gene and the replication origin of NR1. It seems possible that DNA bending in this region might help to orient the replication origin alongside the repA1 gene, which could contribute to the cis-acting character of the RepA1 initiation protein.

This article has been cited by other articles:

• Merino, E., Garciarrubio, A. (2000). The global intrinsic curvature of archaeal and eubacterial genomes is mostly contained in their dinucleotide composition and is probably not an adaptation. Nucleic Acids Res 28: 2431-2438 [Abstract] [Full Text]  
• Praszkier, J., Pittard, A. J. (1999). Role of CIS in Replication of an IncB Plasmid. J. Bacteriol. 181: 2765-2772 [Abstract] [Full Text]  
• del Solar, G., Giraldo, R., Ruiz-Echevarria, M. J., Espinosa, M., Diaz-Orejas, R. (1998). Replication and Control of Circular Bacterial Plasmids. Microbiol. Mol. Biol. Rev. 62: 434-464 [Abstract] [Full Text]