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Molybdenum accumulation in chlD mutants of Escherichia coli.

Department of Nutritional Sciences, University of California, Berkeley 94720.

ABSTRACT

The content of molybdenum in wild-type and chlD cells was measured under a variety of growth conditions to determine if cells with a defective chlD gene were able to accumulate molybdenum. The chlD cells accumulated less molybdenum than wild-type cells did but concentrated molybdenum to a level at least 20-fold higher than the concentration in the culture medium. Molybdenum was present within spheroplasts of chlD cells and was not dialyzable. The chlD cells accumulated as much molybdenum as wild-type cells did when grown in medium containing 0.1 mM molybdate; thus, the capability of incorporation of molybdenum into cellular component(s) was equivalent to that of the wild type under these conditions.

This article has been cited by other articles:

• Rech, S., Wolin, C., Gunsalus, R. P. (1996). Properties of the Periplasmic ModA Molybdate-binding Protein of Escherichia coli. J. Biol. Chem. 271: 2557-2562 [Abstract] [Full Text]