This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bartlett, D H Articles by Silverman, M Search for Related Content PubMed PubMed Citation Articles by Bartlett, D H Articles by Silverman, M

 Previous Article  |  Next Article 

J Bacteriol. 1989 March; 171(3): 1763-1766

research-article

Nucleotide sequence of IS492, a novel insertion sequence causing variation in extracellular polysaccharide production in the marine bacterium Pseudomonas atlantica.

Agouron Institute, La Jolla, California 92037.

ABSTRACT

The complete nucleotide sequence of insertion element IS492, which causes reversible inactivation of extracellular polysaccharide production in the marine bacterium Pseudomonas atlantica, is presented. Insertion of IS492 results in the EPS- phenotype, and excision results in restoration of EPS+. DNA sequencing of the site of insertion in the eps locus showed that insertion of IS492 generates a 5-base-pair repeat and that its excision is precise. IS492 is 1,202 nucleotides in length and contains one large open reading frame encoding a protein of 318 amino acids, a candidate for transposition function. No similarity between IS492 and other transposable elements has been found. Unlike the situation with other insertion sequences, no direct or inverted repeats exist at the termini of IS492.

This article has been cited by other articles:

• van der Woude, M. W., Baumler, A. J. (2004). Phase and Antigenic Variation in Bacteria. Clin. Microbiol. Rev. 17: 581-611 [Abstract] [Full Text]  
• Mitchell, C., Gao, L., Demuth, D. R. (2003). Positive and Negative cis-Acting Regulatory Sequences Control Expression of Leukotoxin in Actinobacillus actinomycetemcomitans 652. Infect. Immun. 71: 5640-5649 [Abstract] [Full Text]  
• Choi, S., Ohta, S., Ohtsubo, E. (2003). A Novel IS Element, IS621, of the IS110/IS492 Family Transposes to a Specific Site in Repetitive Extragenic Palindromic Sequences in Escherichia coli. J. Bacteriol. 185: 4891-4900 [Abstract] [Full Text]  
• Smith, A. B., Siebeling, R. J. (2003). Identification of Genetic Loci Required for Capsular Expression in Vibrio vulnificus. Infect. Immun. 71: 1091-1097 [Abstract] [Full Text]  
• Pellock, B. J., Teplitski, M., Boinay, R. P., Bauer, W. D., Walker, G. C. (2002). A LuxR Homolog Controls Production of Symbiotically Active Extracellular Polysaccharide II by Sinorhizobium meliloti. J. Bacteriol. 184: 5067-5076 [Abstract] [Full Text]  
• Perkins-Balding, D., Duval-Valentin, G., Glasgow, A. C. (1999). Excision of IS492 Requires Flanking Target Sequences and Results in Circle Formation in Pseudoalteromonas atlantica. J. Bacteriol. 181: 4937-4948 [Abstract] [Full Text]  
• Zuppardo, A. B., Siebeling, R. J. (1998). An Epimerase Gene Essential for Capsule Synthesis in Vibrio vulnificus. Infect. Immun. 66: 2601-2606 [Abstract] [Full Text]