This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Xie, W Q Articles by Potts, M Search for Related Content PubMed PubMed Citation Articles by Xie, W Q Articles by Potts, M

research-article

Cyanobacterial RNA polymerase genes rpoC1 and rpoC2 correspond to rpoC of Escherichia coli.

Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg 24061.

ABSTRACT

The DNA-dependent RNA polymerase (ribonucleoside triphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) of cyanobacteria contains a unique core component, gamma, which is absent from the RNA polymerases of other eubacteria (G. J. Schneider, N. E. Tumer, C. Richaud, G. Borbely, and R. Haselkorn, J. Biol. Chem. 262:14633-14639, 1987). We present the complete nucleotide sequence of rpoC1, the gene encoding the gamma subunit, from the heterocystous cyanobacterium Nostoc commune UTEX 584. The derived amino acid sequence of gamma (621 residues) corresponds with the amino-terminal portion of the beta' polypeptide of Escherichia coli RNA polymerase. A second gene in N. commune UTEX 584, rpoC2, encodes a protein which shows correspondence with the carboxy-terminal portion of the E. coli beta' subunit. The rpoBC1C2 genes of N. commune UTEX 584 are present in single copies and are arranged in the order rpoBC1C2, and the coding regions are separated by short AT-rich spacer regions which have the potential to form very stable secondary structures. Our data indicate the occurrence of divergent evolution of structure in the eubacterial DNA-dependent RNA polymerase.

This article has been cited by other articles:

• Imamura, S., Asayama, M., Shirai, M. (2004). In vitro transcription analysis by reconstituted cyanobacterial RNA polymerase: roles of group 1 and 2 sigma factors and a core subunit, RpoC2. GENES CELLS 9: 1175-1187 [Abstract] [Full Text]  
• Valladares, A., Muro-Pastor, A. M., Herrero, A., Flores, E. (2004). The NtcA-Dependent P1 Promoter Is Utilized for glnA Expression in N2-Fixing Heterocysts of Anabaena sp. Strain PCC 7120. J. Bacteriol. 186: 7337-7343 [Abstract] [Full Text]  
• Vogel, J., Axmann, I. M., Herzel, H., Hess, W. R. (2003). Experimental and computational analysis of transcriptional start sites in the cyanobacterium Prochlorococcus MED4. Nucleic Acids Res 31: 2890-2899 [Abstract] [Full Text]  
• Severinov, K., Mustaev, A., Kukarin, A., Muzzin, O., Bass, I., Darst, S. A., Goldfarb, A. (1996). Structural Modules of the Large Subunits of RNA Polymerase. INTRODUCING ARCHAEBACTERIAL AND CHLOROPLAST SPLIT SITES IN THE beta AND beta prime SUBUNITS OF ESCHERICHIA COLI RNA POLYMERASE. J. Biol. Chem. 271: 27969-27974 [Abstract] [Full Text]  
• Weilbaecher, R, Hebron, C, Feng, G, Landick, R (1994). Termination-altering amino acid substitutions in the beta' subunit of Escherichia coli RNA polymerase identify regions involved in RNA chain elongation.. Genes Dev. 8: 2913-2927 [Abstract]  
• Naryshkina, T., Rogulja, D., Golub, L., Severinov, K. (2000). Inter- and Intrasubunit Interactions during the Formation of RNA Polymerase Assembly Intermediate. J. Biol. Chem. 275: 31183-31190 [Abstract] [Full Text]