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J Bacteriol. 1989 April; 171(4): 2216-2218

research-article

The sigma E subunit of Bacillus subtilis RNA polymerase is present in both forespore and mother cell compartments.

Department of Microbiology, University of Texas Health Science Center, San Antonio 78284-7758.

ABSTRACT

Bacillus subtilis cells harvested 3.5 h after the onset of sporulation (t3.5) were fractionated into extracts enriched in either mother cell or forespore components and were analyzed immunologically for sigma E and its precursor protein, P31. We determined by Western blot (immunoblot) analysis that equivalent amounts of P31 and sigma E were present in both mother cell and forespore extracts. This result implies that, although sigma E is not synthesized until a stage in development when the cell is partitioned into progenitor forespore and mother cell compartments, it probably directs the transcription of genes that are expressed in both of these structures.

This article has been cited by other articles:

• Kunkel, B, Kroos, L, Poth, H, Youngman, P, Losick, R (1989). Temporal and spatial control of the mother-cell regulatory gene spoIIID of Bacillus subtilis.. Genes Dev. 3: 1735-1744 [Abstract]