This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Shinomiya, T Articles by Ina, S Search for Related Content PubMed PubMed Citation Articles by Shinomiya, T Articles by Ina, S

research-article

Genetic comparison of bacteriophage PS17 and Pseudomonas aeruginosa R-type pyocin.

Mitsubishi-Kasei Institute of Life Sciences, Tokyo, Japan.

ABSTRACT

PS17 is a bacteriophage of Pseudomonas aeruginosa that is serologically cross-reactive with phage tail-like bacteriocins called R-type pyocins. In addition to having immunological cross-reactivity, certain genes are functionally complementable between PS17 and R-type pyocins. To compare the genetic structures of PS17 and R-type pyocins, a physical map of PS17 genes was constructed by cloning phage DNA fragments on RSF1010-derived vector plasmids. The head and tail gene clusters were tandemly arrayed and together occupied about half of the 41-kilobase-pair PS17 chromosome. With use of these phage clones, the following results were obtained with respect to the genetic relationship between PS17 and R-type pyocins: (i) serological cross-reaction between PS17 and pyocin occurred for the major sheath protein and two components of the fiber, (ii) a certain pyocin mutation was complemented by cloned phage fragments, and (iii) the phage DNA fragment carrying sheath and core tube genes was shown to hybridize to the DNA fragment carrying the pyocin R2 genes.

This article has been cited by other articles:

• Williams, S. R., Gebhart, D., Martin, D. W., Scholl, D. (2008). Retargeting R-Type Pyocins To Generate Novel Bactericidal Protein Complexes. Appl. Environ. Microbiol. 74: 3868-3876 [Abstract] [Full Text]  
• Hernandez-Romero, D., Lucas-Elio, P., Lopez-Serrano, D., Solano, F., Sanchez-Amat, A. (2003). Marinomonas mediterranea is a lysogenic bacterium that synthesizes R-bodies. Microbiology 149: 2679-2686 [Abstract] [Full Text]  
• Jabrane, A., Sabri, A., Compere, P., Jacques, P., Vandenberghe, I., Van Beeumen, J., Thonart, P. (2002). Characterization of Serracin P, a Phage-Tail-Like Bacteriocin, and Its Activity against Erwinia amylovora, the Fire Blight Pathogen. Appl. Environ. Microbiol. 68: 5704-5710 [Abstract] [Full Text]  
• Lee, F. K. N., Dudas, K. C., Hanson, J. A., Nelson, M. B., LoVerde, P. T., Apicella, M. A. (1999). The R-Type Pyocin of Pseudomonas aeruginosa C Is a Bacteriophage Tail-Like Particle That Contains Single-Stranded DNA. Infect. Immun. 67: 717-725 [Abstract] [Full Text]