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J Bacteriol. 1989 May; 171(5): 2689-2696

research-article

Characterization of degP, a gene required for proteolysis in the cell envelope and essential for growth of Escherichia coli at high temperature.

Department of Microbiology and Molecular Genetics, Harvard University Medical School, Boston, Massachusetts 02115.

ABSTRACT

The degP gene, required for proteolysis in the cell envelope of Escherichia coli, maps at approximately 3.5 min on the chromosome. Null mutations in degP result in temperature-sensitive growth. In certain genetic backgrounds, expression of abnormal periplasmic or inner membrane proteins (protein fusions or proteins with internal deletions) enhances the temperature-sensitive phenotype. Such growth defects were used as a selection for cloning the degP gene into Mud4042 and pACYC184 plasmid vectors, and a restriction map was determined. Analysis of deletion and insertion mutations on one of these plasmids showed that the degP gene is approximately 1.5 kilobases in size. The plasmid-encoded DegP protein had an apparent molecular weight of 50,000, as determined by maxicell analysis. Protein fusions between DegP and alkaline phosphatase had high alkaline phosphatase enzymatic activity, indicating that DegP is a periplasmic or membrane protein.

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• Henderson, I. R., Czeczulin, J., Eslava, C., Noriega, F., Nataro, J. P. (1999). Characterization of Pic, a Secreted Protease of Shigella flexneri and Enteroaggregative Escherichia coli. Infect. Immun. 67: 5587-5596 [Abstract] [Full Text]  
• Raivio, T. L., Popkin, D. L., Silhavy, T. J. (1999). The Cpx Envelope Stress Response Is Controlled by Amplification and Feedback Inhibition. J. Bacteriol. 181: 5263-5272 [Abstract] [Full Text]  
• Humphreys, S., Stevenson, A., Bacon, A., Weinhardt, A. B., Roberts, M. (1999). The Alternative Sigma Factor, sigma E, Is Critically Important for the Virulence of Salmonella typhimurium. Infect. Immun. 67: 1560-1568 [Abstract] [Full Text]  
• Henderson, I. R., Owen, P. (1999). The Major Phase-Variable Outer Membrane Protein of Escherichia coli Structurally Resembles the Immunoglobulin A1 Protease Class of Exported Protein and Is Regulated by a Novel Mechanism Involving Dam and OxyR. J. Bacteriol. 181: 2132-2141 [Abstract] [Full Text]  
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• Hu, S.-I., Carozza, M., Klein, M., Nantermet, P., Luk, D., Crowl, R. M. (1998). Human HtrA, an Evolutionarily Conserved Serine Protease Identified as a Differentially Expressed Gene Product in Osteoarthritic Cartilage. J. Biol. Chem. 273: 34406-34412 [Abstract] [Full Text]  
• Suhan, M. L., Hovde, C. J. (1998). Disruption of an Internal Membrane-Spanning Region in Shiga Toxin 1 Reduces Cytotoxicity. Infect. Immun. 66: 5252-5259 [Abstract] [Full Text]  
• Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]  
• Stephenson, K., Harwood, C. R. (1998). Influence of a Cell-Wall-Associated Protease on Production of alpha -Amylase by Bacillus subtilis. Appl. Environ. Microbiol. 64: 2875-2881 [Abstract] [Full Text]  
• Roberts, M., Li, J., Bacon, A., Chatfield, S. (1998). Oral Vaccination against Tetanus: Comparison of the Immunogenicities of Salmonella Strains Expressing Fragment C from the nirB and htrA Promoters. Infect. Immun. 66: 3080-3087 [Abstract] [Full Text]  
• Eslava, C., Navarro-Garcia, F., Czeczulin, J. R., Henderson, I. R., Cravioto, A., Nataro, J. P. (1998). Pet, an Autotransporter Enterotoxin from Enteroaggregative Escherichia coli. Infect. Immun. 66: 3155-3163 [Abstract] [Full Text]  
• van de Poll, M. L. M., van Vugt, M. J. H., Lenferink, A. E. G., van Zoelen, E. J. J. (1998). Identification of the Minimal Requirements for Binding to the Human Epidermal Growth Factor (EGF) Receptor Using Chimeras of Human EGF and an EGF Repeat of Drosophila Notch. J. Biol. Chem. 273: 16075-16081 [Abstract] [Full Text]  
• Cosma, C. L., Crotwell, M. D., Burrows, S. Y., Silhavy, T. J. (1998). . J. Bacteriol. 180: 3120-3130 [Abstract] [Full Text]  
• Betton, J.-M., Sassoon, N., Hofnung, M., Laurent, M. (1998). Degradation versus Aggregation of Misfolded Maltose-binding Protein in the Periplasm of Escherichia coli. J. Biol. Chem. 273: 8897-8902 [Abstract] [Full Text]  
• Miller, K. W., Schamber, R., Chen, Y., Ray, B. (1998). Production of Active Chimeric Pediocin AcH in Escherichia coli in the Absence of Processing and Secretion Genes from the Pediococcus pap Operon. Appl. Environ. Microbiol. 64: 14-20 [Abstract] [Full Text]  
• Connolly, L., Penas, A. D. L., Alba, B. M., Gross, C. A. (1997). The response to extracytoplasmic stress in Escherichia coli is controlled by partially overlapping pathways. Genes Dev. 11: 2012-2021 [Abstract] [Full Text]  
• Pogliano, J, Lynch, A S, Belin, D, Lin, E C, Beckwith, J (1997). Regulation of Escherichia coli cell envelope proteins involved in protein folding and degradation by the Cpx two-component system.. Genes Dev. 11: 1169-1182 [Abstract]  
• Skorko-Glonek, J., Skórko-Glonek, J., Krzewski, K., Zolese, G., Bertoli, E., Tanfani, F. (1997). HtrA Heat Shock Protease Interacts with Phospholipid Membranes and Undergoes Conformational Changes. J. Biol. Chem. 272: 8974-8982 [Abstract] [Full Text]  
• Bullitt, E., Jones, C. H., Striker, R., Soto, G., Jacob-Dubuisson, F., Pinkner, J., Wick, M. J., Makowski, L., Hultgren, S. J. (1996). Development of pilus organelle subassemblies in vitro depends on chaperone uncapping of a beta zipper. Proc. Natl. Acad. Sci. USA 93: 12890-12895 [Abstract] [Full Text]  
• Anaguchi, H., Hiraoka, O., Yamasaki, K., Naito, S., Ota, Y. (1995). Ligand Binding Characteristics of the Carboxyl-terminal Domain of the Cytokine Receptor Homologous Region of the Granulocyte Colony-stimulating Factor Receptor. J. Biol. Chem. 270: 27845-27851 [Abstract] [Full Text]  
• van de Poll, M. L. M., Lenferink, A. E. G., van Vugt, M. J. H., Jacobs, J. J. L., Janssen, J. W. H., Joldersma, M., van Zoelen, E. J. J. (1995). A Single Amino Acid Exchange, Arg-45 to Ala, Generates an Epidermal Growth Factor (EGF) Mutant with High Affinity for the Chicken EGF Receptor. J. Biol. Chem. 270: 22337-22343 [Abstract] [Full Text]  
• Danese, P N, Snyder, W B, Cosma, C L, Davis, L J, Silhavy, T J (1995). The Cpx two-component signal transduction pathway of Escherichia coli regulates transcription of the gene specifying the stress-inducible periplasmic protease, DegP.. Genes Dev. 9: 387-398 [Abstract]  
• Mecsas, J, Rouviere, P E, Erickson, J W, Donohue, T J, Gross, C A (1993). The activity of sigma E, an Escherichia coli heat-inducible sigma-factor, is modulated by expression of outer membrane proteins.. Genes Dev. 7: 2618-2628 [Abstract]  
• Chung, C. (1993). Proteases in Escherichia coli. Science 262: 372-374