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J Bacteriol. 1989 June; 171(6): 2975-2980

research-article

Escherichia coli dnaT gene function is required for pBR322 plasmid replication but not for R1 plasmid replication.

H Masai and K Arai

Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, California 94304.

ABSTRACT

Plasmid pBR322 was unable to replicate in a temperature-sensitive dnaT1 strain at a nonpermissive temperature, whereas a pBR322-derived plasmid carrying the wild-type dnaT+ gene was able to replicate under the same conditions. In contrast to pBR322, plasmid R1 could replicate in the dnaT1 strain at a nonpermissive temperature. In keeping with this finding, in vitro replication of plasmid R1 did not require DnaT protein.

J Bacteriol. 1989 June; 171(6): 2975-2980




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