This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bonam, D Articles by Ludden, P W Search for Related Content PubMed PubMed Citation Articles by Bonam, D Articles by Ludden, P W

research-article

Regulation of carbon monoxide dehydrogenase and hydrogenase in Rhodospirillum rubrum: effects of CO and oxygen on synthesis and activity.

Department of Biochemistry, University of Wisconsin, Madison 53706-1569.

ABSTRACT

Exposure of the photosynthetic bacterium Rhodospirillum rubrum to carbon monoxide led to increased carbon monoxide dehydrogenase and hydrogenase activities due to de novo protein synthesis of both enzymes. Two-dimensional gels of [35S]methionine-pulse-labeled cells showed that induction of CO dehydrogenase synthesis was rapidly initiated (less than 5 min upon exposure to CO) and was inhibited by oxygen. Both CO dehydrogenase and the CO-induced hydrogenase were inactivated by oxygen in vivo and in vitro. In contrast to CO dehydrogenase, the CO-induced hydrogenase was 95% inactivated by heating at 70 degrees C for 5 min. Unlike other hydrogenases, this CO-induced hydrogenase was inhibited only 60% by a 100% CO gas phase.

This article has been cited by other articles:

• Reslewic, S., Zhou, S., Place, M., Zhang, Y., Briska, A., Goldstein, S., Churas, C., Runnheim, R., Forrest, D., Lim, A., Lapidus, A., Han, C. S., Roberts, G. P., Schwartz, D. C. (2005). Whole-Genome Shotgun Optical Mapping of Rhodospirillum rubrum. Appl. Environ. Microbiol. 71: 5511-5522 [Abstract] [Full Text]  
• Rother, M., Metcalf, W. W. (2004). Anaerobic growth of Methanosarcina acetivorans C2A on carbon monoxide: An unusual way of life for a methanogenic archaeon. Proc. Natl. Acad. Sci. USA 101: 16929-16934 [Abstract] [Full Text]  
• Sokolova, T. G., Gonzalez, J. M., Kostrikina, N. A., Chernyh, N. A., Slepova, T. V., Bonch-Osmolovskaya, E. A., Robb, F. T. (2004). Thermosinus carboxydivorans gen. nov., sp. nov., a new anaerobic, thermophilic, carbon-monoxide-oxidizing, hydrogenogenic bacterium from a hot pool of Yellowstone National Park. Int. J. Syst. Evol. Microbiol. 54: 2353-2359 [Abstract] [Full Text]  
• Maness, P.-C., Smolinski, S., Dillon, A. C., Heben, M. J., Weaver, P. F. (2002). Characterization of the Oxygen Tolerance of a Hydrogenase Linked to a Carbon Monoxide Oxidation Pathway in Rubrivivax gelatinosus. Appl. Environ. Microbiol. 68: 2633-2636 [Abstract] [Full Text]  
• Leduc, J., Thorsteinsson, M. V., Gaal, T., Roberts, G. P. (2001). Mapping CooA{middle dot}RNA Polymerase Interactions. IDENTIFICATION OF ACTIVATING REGIONS 2 AND 3 IN CooA, THE CO-SENSING TRANSCRIPTIONAL ACTIVATOR. J. Biol. Chem. 276: 39968-39973 [Abstract] [Full Text]  
• Watt, R. K., Ludden, P. W. (1999). Ni2+ Transport and Accumulation in Rhodospirillum rubrum. J. Bacteriol. 181: 4554-4560 [Abstract] [Full Text]  
• He, Y., Gaal, T., Karls, R., Donohue, T. J., Gourse, R. L., Roberts, G. P. (1999). Transcription Activation by CooA, the CO-sensing Factor from Rhodospirillum rubrum. THE INTERACTION BETWEEN CooA AND THE C-TERMINAL DOMAIN OF THE alpha  SUBUNIT OF RNA POLYMERASE. J. Biol. Chem. 274: 10840-10845 [Abstract] [Full Text]  
• Uchida, T., Ishikawa, H., Takahashi, S., Ishimori, K., Morishima, I., Ohkubo, K., Nakajima, H., Aono, S. (1998). Heme Environmental Structure of CooA Is Modulated by the Target DNA Binding. EVIDENCE FROM RESONANCE RAMAN SPECTROSCOPY AND CO REBINDING KINETICS. J. Biol. Chem. 273: 19988-19992 [Abstract] [Full Text]  
• Watt, R. K., Ludden, P. W. (1998). The Identification, Purification, and Characterization of CooJ. A NICKEL-BINDING PROTEIN THAT IS CO-REGULATED WITH THE Ni-CONTAINING CO DEHYDROGENASE FROM RHODOSPIRILLUM RUBRUM. J. Biol. Chem. 273: 10019-10025 [Abstract] [Full Text]  
• Shelver, D., Kerby, R. L., He, Y., Roberts, G. P. (1997). CooA, a CO-sensing transcription factor from Rhodospirillum rubrum, is a CO-binding heme protein. Proc. Natl. Acad. Sci. USA 94: 11216-11220 [Abstract] [Full Text]  
• He, Y., Shelver, D., Kerby, R. L., Roberts, G. P. (1996). Characterization of a CO-responsive Transcriptional Activator from Rhodospirillum rubrum. J. Biol. Chem. 271: 120-123 [Abstract] [Full Text]