This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Smith, C M Articles by Eisenstadt, E Search for Related Content PubMed PubMed Citation Articles by Smith, C M Articles by Eisenstadt, E

research-article

Identification of a umuDC locus in Salmonella typhimurium LT2.

Laboratory of Toxicology, Harvard University School of Public Health, Boston, Massachusetts 02115.

ABSTRACT

The umuDC operon of Escherichia coli is required for efficient mutagenesis by UV light and many other DNA-damaging agents. The existence of a umuDC analog in Salmonella typhimurium has been questioned. With DNA probes to the E. coli umuD and umuC genes, we detected, by Southern blot hybridization, sequences similar to both of these genes in S. typhimurium LT2. We also confirmed that the presence of cloned E. coli umuD enhances the UV mutability and resistance of S. typhimurium. Our data strongly suggest that S. typhimurium contains a functional umuDC operon.

This article has been cited by other articles:

• Peng, S.-B., Wang, L., Moomaw, J., Peery, R. B., Sun, P.-M., Johnson, R. B., Lu, J., Treadway, P., Skatrud, P. L., Wang, Q. M. (2001). Biochemical Characterization of Signal Peptidase I from Gram-Positive Streptococcus pneumoniae. J. Bacteriol. 183: 621-627 [Abstract] [Full Text]  
• DeMarini, D. M., Shelton, M. L., Abu-Shakra, A., Szakmary, A., Levine, J. G. (1998). Spectra of Spontaneous Frameshift Mutations at the hisD3052 Allele of Salmonella typhimurium in Four DNA Repair Backgrounds. Genetics 149: 17-36 [Abstract] [Full Text]