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J Bacteriol. 1989 July; 171(7): 4083-4084

research-article

Use of the isocitrate dehydrogenase structural gene for attachment of e14 in Escherichia coli K-12.

Department of Biological Chemistry, Milton S. Hershey Medical Center, Pennsylvania 17033.

ABSTRACT

The e14 element appears to be integrated into the Escherichia coli K-12 isocitrate dehydrogenase structural gene (icd). In being integrated, it replaced the last 52 codons of the gene with a closely related sequence. The two versions of the icd gene produce proteins of the same length but differ by 12 base substitutions that would cause two conservative amino acid replacements.

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