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J Bacteriol. 1989 July; 171(7): 4085-4087

research-article

Regulation and role in pathogenicity of Erwinia chrysanthemi 3937 pectin methylesterase.

Laboratoire de Pathologie Végétale, Institut National Agronomique, Paris, France.

ABSTRACT

The gene pem, encoding the pectin methylesterase (PME) of Erwinia chrysanthemi 3937, was cloned and mutagenized by mini-Mu transposable elements. A second gene, pecY, which could act as a negative regulator of PME was found 5' to the pem gene. A PME-E. chrysanthemi derivative inoculate onto Saintpaulia plants was shown to be clearly noninvasive, demonstrating the important role of this enzyme in soft rot disease.

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