This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Misra, R Articles by Benson, S A Search for Related Content PubMed PubMed Citation Articles by Misra, R Articles by Benson, S A

research-article

A novel mutation, cog, which results in production of a new porin protein (OmpG) of Escherichia coli K-12.

Department of Biology, Princeton University, New Jersey 08544-1014.

ABSTRACT

A mutant of Escherichia coli K-12 which produces a new outer membrane protein, OmpG, was isolated and genetically and biochemically characterized. The presence of OmpG allows growth on maltodextrins in the absence of the LamB maltoporin. The data obtained from in vivo growth and uptake experiments suggested that the presence of the OmpG protein results in an increase in outer membrane permeability for small hydrophilic compounds. In light of these findings, we suggest that OmpG is a porinlike protein. The mutation which results in the expression of OmpG has been termed cog (for control of OmpG) and mapped to 29 min on the E. coli chromosome. Diploid analysis shows that the mutant cog-192 allele is recessive for both the Dex+ and OmpG+ phenotypes. We propose that the cog mutation destroys a negative regulatory function and therefore derepresses ompG expression.

This article has been cited by other articles:

• Liang, B., Tamm, L. K. (2007). Structure of outer membrane protein G by solution NMR spectroscopy. Proc. Natl. Acad. Sci. USA 104: 16140-16145 [Abstract] [Full Text]  
• Charlson, E. S., Werner, J. N., Misra, R. (2006). Differential Effects of yfgL Mutation on Escherichia coli Outer Membrane Proteins and Lipopolysaccharide.. J. Bacteriol. 188: 7186-7194 [Abstract] [Full Text]  
• Nikaido, H. (2003). Molecular Basis of Bacterial Outer Membrane Permeability Revisited. Microbiol. Mol. Biol. Rev. 67: 593-656 [Abstract] [Full Text]  
• Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]  
• Fajardo, D. A., Cheung, J., Ito, C., Sugawara, E., Nikaido, H., Misra, R. (1998). Biochemistry and Regulation of a Novel Escherichia coli K-12 Porin Protein, OmpG, Which Produces Unusually Large Channels. J. Bacteriol. 180: 4452-4459 [Abstract] [Full Text]  
• Prilipov, A., Phale, P. S., Koebnik, R., Widmer, C., Rosenbusch, J. P. (1998). Identification and Characterization of Two Quiescent Porin Genes, nmpC and ompN, in Escherichia coli BE. J. Bacteriol. 180: 3388-3392 [Abstract] [Full Text]