This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bilge, S S Articles by Moseley, S L Search for Related Content PubMed PubMed Citation Articles by Bilge, S S Articles by Moseley, S L

research-article

Molecular characterization of a fimbrial adhesin, F1845, mediating diffuse adherence of diarrhea-associated Escherichia coli to HEp-2 cells.

Department of Microbiology, University of Washington, Seattle 98195.

ABSTRACT

A fimbrial adhesin, designated F1845, was found to be responsible for the diffuse HEp-2 cell adherence of a diarrheal Escherichia coli isolate. The genetic determinant of F1845 was cloned, and the order of the genes necessary for production of F1845 was determined by maxicell analysis. Five polypeptides with apparent sizes of 10, 95, 27, 15.5, and 14.3 kilodaltons (kDa) were found to be encoded in that order by the F1845 determinant. The nucleotide sequence of the 14.3-kDa subunit gene was determined and found to share extensive homology in its signal sequence with the gene encoding the structural subunit of the AFA-1 hemagglutinin of a uropathogenic E. coli strain (A. Labigne-Roussel, M.A. Schmidt, W. Walz, and S. Falkow, J. Bacteriol. 162:1285-1292, 1985) but not in the region encoding the mature protein. Southern blot hybridizations indicated that the F1845 determinants are of chromosomal origin. Hybridization studies using a probe from the region encoding the 95-kDa polypeptide indicated that related sequences may be plasmid associated in some strains and chromosomal in others. Additional hybridization studies of E. coli isolates possessing sequence homology to the F1845 determinant suggest that the sequences in the 5' region of the F1845 structural subunit gene are more highly conserved than sequences in the 3' region.

This article has been cited by other articles:

• Hien, B. T. T., Scheutz, F., Cam, P. D., Serichantalergs, O., Huong, T. T., Thu, T. M., Dalsgaard, A. (2008). Diarrheagenic Escherichia coli and Shigella Strains Isolated from Children in a Hospital Case-Control Study in Hanoi, Vietnam. J. Clin. Microbiol. 46: 996-1004 [Abstract] [Full Text]  
• Lievin-Le Moal, V., Sarrazin-Davila, L. E., Servin, A. L. (2007). An Experimental Study and a Randomized, Double-Blind, Placebo-Controlled Clinical Trial to Evaluate the Antisecretory Activity of Lactobacillus acidophilus Strain LB Against Nonrotavirus Diarrhea. Pediatrics 120: e795-e803 [Abstract] [Full Text]  
• Hien, B. T. T., Trang, D. T., Scheutz, F., Cam, P. D., Molbak, K., Dalsgaard, A. (2007). Diarrhoeagenic Escherichia coli and other causes of childhood diarrhoea: a case control study in children living in a wastewater-use area in Hanoi, Vietnam. J Med Microbiol 56: 1086-1096 [Abstract] [Full Text]  
• Charbonneau, M.-E., Berthiaume, F., Mourez, M. (2006). Proteolytic Processing Is Not Essential for Multiple Functions of the Escherichia coli Autotransporter Adhesin Involved in Diffuse Adherence (AIDA-I). J. Bacteriol. 188: 8504-8512 [Abstract] [Full Text]  
• Korotkova, N., Le Trong, I., Samudrala, R., Korotkov, K., Van Loy, C. P., Bui, A.-L., Moseley, S. L., Stenkamp, R. E. (2006). Crystal Structure and Mutational Analysis of the DaaE Adhesin of Escherichia coli. J. Biol. Chem. 281: 22367-22377 [Abstract] [Full Text]  
• Vidal, M., Kruger, E., Duran, C., Lagos, R., Levine, M., Prado, V., Toro, C., Vidal, R. (2005). Single Multiplex PCR Assay To Identify Simultaneously the Six Categories of Diarrheagenic Escherichia coli Associated with Enteric Infections. J. Clin. Microbiol. 43: 5362-5365 [Abstract] [Full Text]  
• Servin, A. L. (2005). Pathogenesis of Afa/Dr Diffusely Adhering Escherichia coli. Clin. Microbiol. Rev. 18: 264-292 [Abstract] [Full Text]  
• Lopes, L. M., Fabbricotti, S. H., Ferreira, A. J. P., Kato, M. A. M. F., Michalski, J., Scaletsky, I. C. A. (2005). Heterogeneity among Strains of Diffusely Adherent Escherichia coli Isolated in Brazil. J. Clin. Microbiol. 43: 1968-1972 [Abstract] [Full Text]  
• Torres, A. G., Zhou, X., Kaper, J. B. (2005). Adherence of Diarrheagenic Escherichia coli Strains to Epithelial Cells. Infect. Immun. 73: 18-29 [Full Text]  
• Aranda, K. R. S., Fagundes-Neto, U., Scaletsky, I. C. A. (2004). Evaluation of Multiplex PCRs for Diagnosis of Infection with Diarrheagenic Escherichia coli and Shigella spp.. J. Clin. Microbiol. 42: 5849-5853 [Abstract] [Full Text]  
• Pettigrew, D., Anderson, K. L., Billington, J., Cota, E., Simpson, P., Urvil, P., Rabuzin, F., Roversi, P., Nowicki, B., du Merle, L., Le Bouguenec, C., Matthews, S., Lea, S. M. (2004). High Resolution Studies of the Afa/Dr Adhesin DraE and Its Interaction with Chloramphenicol. J. Biol. Chem. 279: 46851-46857 [Abstract] [Full Text]  
• Gartner, J. F., Schmidt, M. A. (2004). Comparative Analysis of Locus of Enterocyte Effacement Pathogenicity Islands of Atypical Enteropathogenic Escherichia coli. Infect. Immun. 72: 6722-6728 [Abstract] [Full Text]  
• Brest, P., Betis, F., Cuburu, N., Selva, E., Herrant, M., Servin, A., Auberger, P., Hofman, P. (2004). Increased Rate of Apoptosis and Diminished Phagocytic Ability of Human Neutrophils Infected with Afa/Dr Diffusely Adhering Escherichia coli Strains. Infect. Immun. 72: 5741-5749 [Abstract] [Full Text]  
• Bernier-Febreau, C., du Merle, L., Turlin, E., Labas, V., Ordonez, J., Gilles, A.-M., Le Bouguenec, C. (2004). Use of Deoxyribose by Intestinal and Extraintestinal Pathogenic Escherichia coli Strains: a Metabolic Adaptation Involved in Competitiveness. Infect. Immun. 72: 6151-6156 [Abstract] [Full Text]  
• Hudault, S., Spiller, O. B., Morgan, B. P., Servin, A. L. (2004). Human Diffusely Adhering Escherichia coli Expressing Afa/Dr Adhesins That Use Human CD55 (Decay-Accelerating Factor) as a Receptor Does Not Bind the Rodent and Pig Analogues of CD55. Infect. Immun. 72: 4859-4863 [Abstract] [Full Text]  
• Kansau, I., Berger, C., Hospital, M., Amsellem, R., Nicolas, V., Servin, A. L., Bernet-Camard, M.-F. (2004). Zipper-Like Internalization of Dr-Positive Escherichia coli by Epithelial Cells Is Preceded by an Adhesin-Induced Mobilization of Raft-Associated Molecules in the Initial Step of Adhesion. Infect. Immun. 72: 3733-3742 [Abstract] [Full Text]  
• Zalewska, B., Piatek, R., Konopa, G., Nowicki, B., Nowicki, S., Kur, J. (2003). Chimeric Dr Fimbriae with a Herpes Simplex Virus Type 1 Epitope as a Model for a Recombinant Vaccine. Infect. Immun. 71: 5505-5513 [Abstract] [Full Text]  
• Okeke, I. N., Ojo, O., Lamikanra, A., Kaper, J. B. (2003). Etiology of Acute Diarrhea in Adults in Southwestern Nigeria. J. Clin. Microbiol. 41: 4525-4530 [Abstract] [Full Text]  
• Valentiner-Branth, P., Steinsland, H., Fischer, T. K., Perch, M., Scheutz, F., Dias, F., Aaby, P., Molbak, K., Sommerfelt, H. (2003). Cohort Study of Guinean Children: Incidence, Pathogenicity, Conferred Protection, and Attributable Risk for Enteropathogens during the First 2 Years of Life. J. Clin. Microbiol. 41: 4238-4245 [Abstract] [Full Text]  
• Beutin, L., Marches, O., Bettelheim, K. A., Gleier, K., Zimmermann, S., Schmidt, H., Oswald, E. (2003). HEp-2 Cell Adherence, Actin Aggregation, and Intimin Types of Attaching and Effacing Escherichia coli Strains Isolated from Healthy Infants in Germany and Australia. Infect. Immun. 71: 3995-4002 [Abstract] [Full Text]  
• Laarmann, S., Schmidt, M. A. (2003). The Escherichia coli AIDA autotransporter adhesin recognizes an integral membrane glycoprotein as receptor. Microbiology 149: 1871-1882 [Abstract] [Full Text]  
• Betis, F., Brest, P., Hofman, V., Guignot, J., Kansau, I., Rossi, B., Servin, A., Hofman, P. (2003). Afa/Dr Diffusely Adhering Escherichia coli Infection in T84 Cell Monolayers Induces Increased Neutrophil Transepithelial Migration, Which in Turn Promotes Cytokine-Dependent Upregulation of Decay-Accelerating Factor (CD55), the Receptor for Afa/Dr Adhesins. Infect. Immun. 71: 1774-1783 [Abstract] [Full Text]  
• Betis, F., Brest, P., Hofman, V., Guignot, J., Bernet-Camard, M.-F., Rossi, B., Servin, A., Hofman, P. (2003). The Afa/Dr Adhesins of Diffusely Adhering Escherichia coli Stimulate Interleukin-8 Secretion, Activate Mitogen-Activated Protein Kinases, and Promote Polymorphonuclear Transepithelial Migration in T84 Polarized Epithelial Cells. Infect. Immun. 71: 1068-1074 [Abstract] [Full Text]  
• Blanc-Potard, A.-B., Tinsley, C., Scaletsky, I., Le Bouguenec, C., Guignot, J., Servin, A. L., Nassif, X., Bernet-Camard, M.-F. (2002). Representational Difference Analysis between Afa/Dr Diffusely Adhering Escherichia coli and Nonpathogenic E. coli K-12. Infect. Immun. 70: 5503-5511 [Abstract] [Full Text]  
• White-Ziegler, C. A., Black, A. M., Eliades, S. H., Young, S., Porter, K. (2002). The N-Acetyltransferase RimJ Responds to Environmental Stimuli To Repress pap Fimbrial Transcription in Escherichia coli. J. Bacteriol. 184: 4334-4342 [Abstract] [Full Text]  
• Bernier, C., Gounon, P., Le Bouguenec, C. (2002). Identification of an Aggregative Adhesion Fimbria (AAF) Type III-Encoding Operon in Enteroaggregative Escherichia coli as a Sensitive Probe for Detecting the AAF-Encoding Operon Family. Infect. Immun. 70: 4302-4311 [Abstract] [Full Text]  
• Lievin-Le Moal, V, Amsellem, R, Servin, A L, Coconnier, M-H (2002). Lactobacillus acidophilus (strain LB) from the resident adult human gastrointestinal microflora exerts activity against brush border damage promoted by a diarrhoeagenic Escherichia coli in human enterocyte-like cells. Gut 50: 803-811 [Abstract] [Full Text]  
• Keller, R., Ordonez, J. G., de Oliveira, R. R., Trabulsi, L. R., Baldwin, T. J., Knutton, S. (2002). Afa, a Diffuse Adherence Fibrillar Adhesin Associated with Enteropathogenic Escherichia coli. Infect. Immun. 70: 2681-2689 [Abstract] [Full Text]  
• Van Loy, C. P., Sokurenko, E. V., Moseley, S. L. (2002). The Major Structural Subunits of Dr and F1845 Fimbriae Are Adhesins. Infect. Immun. 70: 1694-1702 [Abstract] [Full Text]  
• Scaletsky, I. C. A., Fabbricotti, S. H., Aranda, K. R., Morais, M. B., Fagundes-Neto, U. (2002). Comparison of DNA Hybridization and PCR Assays for Detection of Putative Pathogenic Enteroadherent Escherichia coli. J. Clin. Microbiol. 40: 1254-1258 [Abstract] [Full Text]  
• Scaletsky, I. C. A., Fabbricotti, S. H., Carvalho, R. L. B., Nunes, C. R., Maranhao, H. S., Morais, M. B., Fagundes-Neto, U. (2002). Diffusely Adherent Escherichia coli as a Cause of Acute Diarrhea in Young Children in Northeast Brazil: a Case-Control Study. J. Clin. Microbiol. 40: 645-648 [Abstract] [Full Text]  
• Sokurenko, E. V., Tchesnokova, V., Yeung, A. T., Oleykowski, C. A., Trintchina, E., Hughes, K. T., Rashid, R. A., Brint, J. M., Moseley, S. L., Lory, S. (2001). Detection of simple mutations and polymorphisms in large genomic regions. Nucleic Acids Res 29: e111-e111 [Abstract] [Full Text]  
• Valentiner-Branth, P., Steinsland, H., Santos, G., Perch, M., Begtrup, K., Bhan, M. K, Dias, F., Aaby, P., Sommerfelt, H., Molbak, K. (2001). Community-based controlled trial of dietary management of children with persistent diarrhea: sustained beneficial effect on ponderal and linear growth. Am. J. Clin. Nutr. 73: 968-974 [Abstract] [Full Text]  
• Le Bouguénec, C., Lalioui, L., du Merle, L., Jouve, M., Courcoux, P., Bouzari, S., Selvarangan, R., Nowicki, B. J., Germani, Y., Andremont, A., Gounon, P., Garcia, M.-I. (2001). Characterization of AfaE Adhesins Produced by Extraintestinal and Intestinal Human Escherichia coli Isolates: PCR Assays for Detection of Afa Adhesins That Do or Do Not Recognize Dr Blood Group Antigens. J. Clin. Microbiol. 39: 1738-1745 [Abstract] [Full Text]  
• Guignot, J., Bernet-Camard, M.-F., Pous, C., Plancon, L., Le Bouguenec, C., Servin, A. L. (2001). Polarized Entry of Uropathogenic Afa/Dr Diffusely Adhering Escherichia coli Strain IH11128 into Human Epithelial Cells: Evidence for {alpha}5{beta}1 Integrin Recognition and Subsequent Internalization through a Pathway Involving Caveolae and Dynamic Unstable Microtubules. Infect. Immun. 69: 1856-1868 [Abstract] [Full Text]  
• Niewerth, U., Frey, A., Voss, T., Le Bouguenec, C., Baljer, G., Franke, S., Schmidt, M. A. (2001). The AIDA Autotransporter System Is Associated with F18 and Stx2e in Escherichia coli Isolates from Pigs Diagnosed with Edema Disease and Postweaning Diarrhea. CVI 8: 143-149 [Abstract] [Full Text]  
• Guignot, J., Breard, J., Bernet-Camard, M.-F., Peiffer, I., Nowicki, B. J., Servin, A. L., Blanc-Potard, A.-B. (2000). Pyelonephritogenic Diffusely Adhering Escherichia coli EC7372 Harboring Dr-II Adhesin Carries Classical Uropathogenic Virulence Genes and Promotes Cell Lysis and Apoptosis in Polarized Epithelial Caco-2/TC7 Cells. Infect. Immun. 68: 7018-7027 [Abstract] [Full Text]  
• White-Ziegler, C. A., Villapakkam, A., Ronaszeki, K., Young, S. (2000). H-NS Controls pap and daa Fimbrial Transcription in Escherichia coli in Response to Multiple Environmental Cues. J. Bacteriol. 182: 6391-6400 [Abstract] [Full Text]  
• Lievin, V, Peiffer, I, Hudault, S, Rochat, F, Brassart, D, Neeser, J-R, Servin, A L (2000). Bifidobacterium strains from resident infant human gastrointestinal microflora exert antimicrobial activity. Gut 47: 646-652 [Abstract] [Full Text]  
• Peiffer, I., Guignot, J., Barbat, A., Carnoy, C., Moseley, S. L., Nowicki, B. J., Servin, A. L., Bernet-Camard, M.-F. (2000). Structural and Functional Lesions in Brush Border of Human Polarized Intestinal Caco-2/TC7 Cells Infected by Members of the Afa/Dr Diffusely Adhering Family of Escherichia coli. Infect. Immun. 68: 5979-5990 [Abstract] [Full Text]  
• Schultsz, C., van den Ende, J., Cobelens, F., Vervoort, T., van Gompel, A., Wetsteyn, J. C. F. M., Dankert, J. (2000). Diarrheagenic Escherichia coli and Acute and Persistent Diarrhea in Returned Travelers. J. Clin. Microbiol. 38: 3550-3554 [Abstract] [Full Text]  
• Johnson, J. R., O'Bryan, T. T., Low, D. A., Ling, G., Delavari, P., Fasching, C., Russo, T. A., Carlino, U., Stell, A. L. (2000). Evidence of Commonality between Canine and Human Extraintestinal Pathogenic Escherichia coli Strains That Express papG Allele III. Infect. Immun. 68: 3327-3336 [Abstract] [Full Text]  
• Peiffer, I., Blanc-Potard, A.-B., Bernet-Camard, M.-F., Guignot, J., Barbat, A., Servin, A. L. (2000). Afa/Dr Diffusely Adhering Escherichia coli C1845 Infection Promotes Selective Injuries in the Junctional Domain of Polarized Human Intestinal Caco-2/TC7 Cells. Infect. Immun. 68: 3431-3442 [Abstract] [Full Text]  
• Guignot, J., Peiffer, I., Bernet-Camard, M.-F., Lublin, D. M., Carnoy, C., Moseley, S. L., Servin, A. L. (2000). Recruitment of CD55 and CD66e Brush Border-Associated Glycosylphosphatidylinositol-Anchored Proteins by Members of the Afa/Dr Diffusely Adhering Family of Escherichia coli That Infect the Human Polarized Intestinal Caco-2/TC7 Cells. Infect. Immun. 68: 3554-3563 [Abstract] [Full Text]  
• Okeke, I. N., Lamikanra, A., Steinrück, H., Kaper, J. B. (2000). Characterization of Escherichia coli Strains from Cases of Childhood Diarrhea in Provincial Southwestern Nigeria. J. Clin. Microbiol. 38: 7-12 [Abstract] [Full Text]  
• Lalioui, L., Jouve, M., Gounon, P., Le Bouguenec, C. (1999). Molecular Cloning and Characterization of the afa-7 and afa-8 Gene Clusters Encoding Afimbrial Adhesins in Escherichia coli Strains Associated with Diarrhea or Septicemia in Calves. Infect. Immun. 67: 5048-5059 [Abstract] [Full Text]  
• Boudeau, J., Glasser, A.-L., Masseret, E., Joly, B., Darfeuille-Michaud, A. (1999). Invasive Ability of an Escherichia coli Strain Isolated from the Ileal Mucosa of a Patient with Crohn's Disease. Infect. Immun. 67: 4499-4509 [Abstract] [Full Text]  
• Goluszko, P., Selvarangan, R., Popov, V., Pham, T., Wen, J. W., Singhal, J. (1999). Decay-Accelerating Factor and Cytoskeleton Redistribution Pattern in HeLa Cells Infected with Recombinant Escherichia coli Strains Expressing Dr Family of Adhesins. Infect. Immun. 67: 3989-3997 [Abstract] [Full Text]  
• Scaletsky, I. C. A., Pedroso, M. Z., Oliva, C. A. G., Carvalho, R. L. B., Morais, M. B., Fagundes-Neto, U. (1999). A Localized Adherence-Like Pattern as a Second Pattern of Adherence of Classic Enteropathogenic Escherichia coli to HEp-2 Cells That Is Associated with Infantile Diarrhea. Infect. Immun. 67: 3410-3415 [Abstract] [Full Text]  
• Elias, W. P. Jr., Czeczulin, J. R., Henderson, I. R., Trabulsi, L. R., Nataro, J. P. (1999). Organization of Biogenesis Genes for Aggregative Adherence Fimbria II Defines a Virulence Gene Cluster in Enteroaggregative Escherichia coli. J. Bacteriol. 181: 1779-1785 [Abstract] [Full Text]  
• Marsh, J. W., Taylor, R. K. (1999). Genetic and Transcriptional Analyses of the Vibrio cholerae Mannose-Sensitive Hemagglutinin Type 4 Pilus Gene Locus. J. Bacteriol. 181: 1110-1117 [Abstract] [Full Text]  
• Le Bouguenec;, C., Nataro, J. P., Kaper, J. B. (1999). Diarrhea-Associated Diffusely Adherent Escherichia coli. Clin. Microbiol. Rev. 12: 180-181 [Full Text]  
• Gomes, T. A. T., Vieira, M. A. M., Abe, C. M., Rodrigues, D., Griffin, P. M., Ramos, S. R. T. S. (1998). Adherence Patterns and Adherence-Related DNA Sequences in Escherichia coli Isolates from Children with and without Diarrhea in Sao Paulo City, Brazil. J. Clin. Microbiol. 36: 3609-3613 [Abstract] [Full Text]  
• Peiffer, I., Servin, A. L., Bernet-Camard, M.-F. (1998). Piracy of Decay-Accelerating Factor (CD55) Signal Transduction by the Diffusely Adhering Strain Escherichia coli C1845 Promotes Cytoskeletal F-Actin Rearrangements in Cultured Human Intestinal INT407 Cells. Infect. Immun. 66: 4036-4042 [Abstract] [Full Text]  
• Ismaili, A., McWhirter, E., Handelsman, M. Y. C., Brunton, J. L., Sherman, P. M. (1998). Divergent Signal Transduction Responses to Infection with Attaching and Effacing Escherichia coli. Infect. Immun. 66: 1688-1696 [Abstract] [Full Text]  
• Beinke, C., Laarmann, S., Wachter, C., Karch, H., Greune, L., Schmidt, M. A. (1998). Diffusely Adhering Escherichia coli Strains Induce Attaching and Effacing Phenotypes and Secrete Homologs of Esp Proteins. Infect. Immun. 66: 528-539 [Abstract] [Full Text]  
• Keller, R., Pedroso, M. Z., Ritchmann, R., Silva, R. M. (1998). Occurrence of Virulence-Associated Properties in Enterobacter cloacae. Infect. Immun. 66: 645-649 [Abstract] [Full Text]  
• Nataro, J. P., Kaper, J. B. (1998). Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 11: 142-201 [Abstract] [Full Text]