This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Smith, L T Articles by Smith, G M Search for Related Content PubMed PubMed Citation Articles by Smith, L T Articles by Smith, G M

research-article

An osmoregulated dipeptide in stressed Rhizobium meliloti.

Plant Growth Laboratory, University of California, Davis 95616.

ABSTRACT

One common mechanism of cellular adaptation to osmotic stress is the accumulation of organic solutes in the cytosol. We have used natural-abundance 13C nuclear magnetic resonance to identify all organic solutes that accumulate to significant levels in Rhizobium meliloti. Our studies led to the discovery of a new dipeptide, N-acetylglutaminylglutamine amide (NAGGN), which is accumulated during osmotic stress. Only rarely have peptides been shown to function in bacteria, and furthermore, this is the first example of a peptide playing a role in osmoregulation. Evidence for the biological role of NAGGN in osmotic-stress protection is presented.

This article has been cited by other articles:

• Vriezen, J. A. C., de Bruijn, F. J., Nusslein, K. (2007). Responses of Rhizobia to Desiccation in Relation to Osmotic Stress, Oxygen, and Temperature. Appl. Environ. Microbiol. 73: 3451-3459 [Full Text]  
• Barra, L., Fontenelle, C., Ermel, G., Trautwetter, A., Walker, G. C., Blanco, C. (2006). Interrelations between Glycine Betaine Catabolism and Methionine Biosynthesis in Sinorhizobium meliloti Strain 102F34.. J. Bacteriol. 188: 7195-7204 [Abstract] [Full Text]  
• Miller-Williams, M., Loewen, P. C., Oresnik, I. J. (2006). Isolation of salt-sensitive mutants of Sinorhizobium meliloti strain Rm1021. Microbiology 152: 2049-2059 [Abstract] [Full Text]  
• Angelidis, A. S., Smith, G. M. (2003). Role of the Glycine Betaine and Carnitine Transporters in Adaptation of Listeria monocytogenes to Chill Stress in Defined Medium. Appl. Environ. Microbiol. 69: 7492-7498 [Abstract] [Full Text]  
• Angelidis, A. S., Smith, G. M. (2003). Three Transporters Mediate Uptake of Glycine Betaine and Carnitine by Listeria monocytogenes in Response to Hyperosmotic Stress. Appl. Environ. Microbiol. 69: 1013-1022 [Abstract] [Full Text]  
• Angelidis, A. S., Smith, L. T., Hoffman, L. M., Smith, G. M. (2002). Identification of OpuC as a Chill-Activated and Osmotically Activated Carnitine Transporter in Listeria monocytogenes. Appl. Environ. Microbiol. 68: 2644-2650 [Abstract] [Full Text]  
• Zahran, H. H. (1999). Rhizobium-Legume Symbiosis and Nitrogen Fixation under Severe Conditions and in an Arid Climate. Microbiol. Mol. Biol. Rev. 63: 968-989 [Abstract] [Full Text]  
• Amezaga, M.-R., Booth, I. R. (1999). Osmoprotection of Escherichia coli by Peptone Is Mediated by the Uptake and Accumulation of Free Proline but Not of Proline-Containing Peptides. Appl. Environ. Microbiol. 65: 5272-5278 [Abstract] [Full Text]  
• Boncompagni, E., Østerås, M., Poggi, M.-C., le Rudulier, D. (1999). Occurrence of Choline and Glycine Betaine Uptake and Metabolism in the Family Rhizobiaceae and Their Roles in Osmoprotection. Appl. Environ. Microbiol. 65: 2072-2077 [Abstract] [Full Text]  
• Gouffi, K., Pica, N., Pichereau, V., Blanco, C. (1999). Disaccharides as a New Class of Nonaccumulated Osmoprotectants for Sinorhizobium meliloti. Appl. Environ. Microbiol. 65: 1491-1500 [Abstract] [Full Text]  
• Gouffi, K., Pichereau, V., Rolland, J.-P., Thomas, D., Bernard, T., Blanco, C. (1998). Sucrose Is a Nonaccumulated Osmoprotectant in Sinorhizobium meliloti. J. Bacteriol. 180: 5044-5051 [Abstract] [Full Text]  
• Pichereau, V., Pocard, J.-A., Hamelin, J., Blanco, C., Bernard, T. (1998). Differential Effects of Dimethylsulfoniopropionate, Dimethylsulfonioacetate, and Other S-Methylated Compounds on the Growth of Sinorhizobium meliloti at Low and High Osmolarities. Appl. Environ. Microbiol. 64: 1420-1429 [Abstract] [Full Text]