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J Bacteriol. 1990 October; 172(10): 5686-5689

research-article

Pyrophosphatase is essential for growth of Escherichia coli.

Laboratoire de Biochimie, 240 du Centre National de la Recherche Scientifique, Ecole Polytechnique, Palaiseau, France.

ABSTRACT

The ppa gene for inorganic pyrophosphatase is essential for the growth of Escherichia coli. A recombinant with a chromosomal ppa::Kanr lesion and a temperature-sensitive replicon with a ppa+ gene showed a temperature-sensitive growth phenotype, and a mutant with the sole ppa+ gene under control of the lac promoter showed inducer-dependent growth. When the lacp-ppa mutant was subcultured without inducer, the pyrophosphatase level decreased, the PPi level increased, and growth stopped. Cellular PPi reached 16 mM about 6 h after growth arrest without loss of cell viability.

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