This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Menou, G Articles by Lereclus, D Search for Related Content PubMed PubMed Citation Articles by Menou, G Articles by Lereclus, D

 Previous Article  |  Next Article 

J Bacteriol. 1990 December; 172(12): 6689-6696

research-article

Structural and genetic organization of IS232, a new insertion sequence of Bacillus thuringiensis.

Département des Biotechnologies, URA 1300 Centre National de la Recherche Scientifique, Institut Pasteur, Paris, France.

ABSTRACT

In the Bacillus thuringiensis strains toxic for the lepidopteran larvae, the delta-endotoxin genes cryIA are frequently found within a composite transposonlike structure flanked by two inverted repeat sequences. We report that these elements are true insertion sequences and designate them IS232. IS232 is a 2,184-bp element and is delimited by two imperfect inverted repeats (28 of 37 bp are identical). Two adjacent open reading frames, overlapping for three codons, span almost the entire sequence of IS232. The potential encoded polypeptides of 50 and 30-kDa are homologous to the IstA and IstB proteins of the gram-negative insertion sequence IS21. The N-terminal part of the 50-kDa polypeptide contains a helix-turn-helix DNA-binding motif. The junctions at the insertion sites of three IS232 elements were analyzed. Each case was different, with 0, 4, or 6 bp of the target DNA being duplicated. Transposition of IS232 in Escherichia coli was demonstrated by using a genetic marker inserted upstream of the two open reading frames.

This article has been cited by other articles:

• Wang, Y., Wang, G.-R., Shoemaker, N. B., Whitehead, T. R., Salyers, A. A. (2005). Distribution of the ermG Gene among Bacterial Isolates from Porcine Intestinal Contents. Appl. Environ. Microbiol. 71: 4930-4934 [Abstract] [Full Text]  
• Nirdnoy, W., Mason, C. J., Guerry, P. (2005). Mosaic Structure of a Multiple-Drug-Resistant, Conjugative Plasmid from Campylobacter jejuni. Antimicrob. Agents Chemother. 49: 2454-2459 [Abstract] [Full Text]  
• Tomita, H., Tanimoto, K., Hayakawa, S., Morinaga, K., Ezaki, K., Oshima, H., Ike, Y. (2003). Highly Conjugative pMG1-Like Plasmids Carrying Tn1546-Like Transposons That Encode Vancomycin Resistance in Enterococcus faecium. J. Bacteriol. 185: 7024-7028 [Abstract] [Full Text]  
• Hashimoto, M., Fukui, M., Hayano, K., Hayatsu, M. (2002). Nucleotide Sequence and Genetic Structure of a Novel Carbaryl Hydrolase Gene (cehA) from Rhizobium sp. Strain AC100. Appl. Environ. Microbiol. 68: 1220-1227 [Abstract] [Full Text]  
• Ogawa, N., Miyashita, K. (1999). The Chlorocatechol-Catabolic Transposon Tn5707 of Alcaligenes eutrophus NH9, Carrying a Gene Cluster Highly Homologous to That in the 1,2,4-Trichlorobenzene-Degrading Bacterium Pseudomonas sp. Strain P51, Confers the Ability To Grow on 3-Chlorobenzoate. Appl. Environ. Microbiol. 65: 724-731 [Abstract] [Full Text]  
• Schnepf, E., Crickmore, N., Van Rie, J., Lereclus, D., Baum, J., Feitelson, J., Zeigler, D. R., Dean, D. H. (1998). Bacillus thuringiensis and Its Pesticidal Crystal Proteins. Microbiol. Mol. Biol. Rev. 62: 775-806 [Abstract] [Full Text]