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J Bacteriol. 1990 December; 172(12): 6736-6740

research-article

Tn10-derived transposons active in Bacillus subtilis.

Laboratoire de Génétique Microbienne, Institut National de Recherche Agronomique--Domaine de Vilvert, Jouy en Josas, France.

ABSTRACT

Small derivatives of the Escherichia coli transposon Tn10, comprising IS10 ends and a chloramphenicol resistance gene, were introduced in Bacillus subtilis on a thermosensitive plasmid, pE194. In the presence of the Tn10 transposase gene fused to signals functional in B. subtilis, these derivatives transposed with a frequency of 10(-6) per element per generation. They had no highly preferred insertion site or region, as judged by restriction analysis of the chromosomal DNA, and generated auxotrophic and sporulation-deficient mutants with a frequency of about 1%. These results suggest that Tn10 derivatives might be a useful genetic tool in B. subtilis and possibly other gram-positive microorganisms.

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