This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hallenbeck, P L Articles by Kaplan, S Search for Related Content PubMed PubMed Citation Articles by Hallenbeck, P L Articles by Kaplan, S

 Previous Article  |  Next Article 

J Bacteriol. 1990 April; 172(4): 1749-1761

research-article

Phosphoribulokinase activity and regulation of CO2 fixation critical for photosynthetic growth of Rhodobacter sphaeroides.

Department of Microbiology, University of Texas Medical School, Houston 77225.

ABSTRACT

The Rhodobacter sphaeroides genome contains two unlinked genetic regions each encoding numerous proteins involved in CO2 fixation which include phosphoribulokinases (prkA and prkB), ribulose 1,5-bisphosphate carboxylase/oxygenase (rbcLS and rbcR) (P. L. Hallenbeck and S. Kaplan, Photosynth. Res. 19:63-71, 1988; F. R. Tabita, Microbiol. Rev. 52:155-189, 1988), and two open reading frames linked to rbcLS and rbcR, namely, cfxA and cfxB, respectively (P. L. Hallenbeck, R. Lerchen, P. Hessler, and S. Kaplan, J. Bacteriol. 172:1736-1748). In this study, we examined the unique role(s) of each phosphoribulokinase activity in the regulation of CO2 fixation. Strains were constructed which contain null mutations in prkA and/or prkB. Studies utilizing these strains suggested that CO2 fixation plays an essential role in attaining the cellular redox balance necessary for photoheterotrophic growth. The presence of an external electron acceptor can negate the requirement for CO2 for photoheterotrophic growth. Each form of phosphoribulokinase and ribulose 1,5-bisphosphate carboxylase/oxygenase was shown to have distinct roles in CO2 metabolism when cells were exposed to extremes in CO2 levels. Evidence is also presented which unequivocally demonstrated that regulation of the expression of the enzymes involved in CO2 metabolism is effective at the transcriptional level. Although the two regions of the DNA involved in CO2 fixation are physically unlinked, each region of the DNA can have a profound effect on the expression of the other region of the DNA.

This article has been cited by other articles:

• Poggio, S., Abreu-Goodger, C., Fabela, S., Osorio, A., Dreyfus, G., Vinuesa, P., Camarena, L. (2007). A Complete Set of Flagellar Genes Acquired by Horizontal Transfer Coexists with the Endogenous Flagellar System in Rhodobacter sphaeroides. J. Bacteriol. 189: 3208-3216 [Abstract] [Full Text]  
• Toyoda, K., Yoshizawa, Y., Arai, H., Ishii, M., Igarashi, Y. (2005). The role of two CbbRs in the transcriptional regulation of three ribulose-1,5-bisphosphate carboxylase/oxygenase genes in Hydrogenovibrio marinus strain MH-110. Microbiology 151: 3615-3625 [Abstract] [Full Text]  
• Tavano, C. L., Podevels, A. M., Donohue, T. J. (2005). Identification of Genes Required for Recycling Reducing Power during Photosynthetic Growth. J. Bacteriol. 187: 5249-5258 [Abstract] [Full Text]  
• Choudhary, M., Fu, Y.-X., Mackenzie, C., Kaplan, S. (2004). DNA Sequence Duplication in Rhodobacter sphaeroides 2.4.1: Evidence of an Ancient Partnership between Chromosomes I and II. J. Bacteriol. 186: 2019-2027 [Abstract] [Full Text]  
• Roh, J. H., Smith, W. E., Kaplan, S. (2004). Effects of Oxygen and Light Intensity on Transcriptome Expression in Rhodobacter sphaeroides 2.4.1: REDOX ACTIVE GENE EXPRESSION PROFILE. J. Biol. Chem. 279: 9146-9155 [Abstract] [Full Text]  
• Dubbs, J. M., Tabita, F. R. (2003). Interactions of the cbbII Promoter-Operator Region with CbbR and RegA (PrrA) Regulators Indicate Distinct Mechanisms to Control Expression of the Two cbb Operons of Rhodobacter sphaeroides. J. Biol. Chem. 278: 16443-16450 [Abstract] [Full Text]  
• Smith, S. A., Tabita, F. R. (2002). Up-Regulated Expression of the cbbI and cbbII Operons during Photoheterotrophic Growth of a Ribulose 1,5-Bisphosphate Carboxylase-Oxygenase Deletion Mutant of Rhodobacter sphaeroides. J. Bacteriol. 184: 6721-6724 [Abstract] [Full Text]  
• Tichi, M. A., Tabita, F. R. (2002). Metabolic Signals That Lead to Control of CBB Gene Expression in Rhodobacter capsulatus. J. Bacteriol. 184: 1905-1915 [Abstract] [Full Text]  
• Tichi, M. A., Meijer, W. G., Tabita, F. R. (2001). Complex I and Its Involvement in Redox Homeostasis and Carbon and Nitrogen Metabolism in Rhodobacter capsulatus. J. Bacteriol. 183: 7285-7294 [Abstract] [Full Text]  
• Tichi, M. A., Tabita, F. R. (2001). Interactive Control of Rhodobacter capsulatus Redox-Balancing Systems during Phototrophic Metabolism. J. Bacteriol. 183: 6344-6354 [Abstract] [Full Text]  
• Choudhary, M., Kaplan, S. (2000). DNA sequence analysis of the photosynthesis region of Rhodobacter sphaeroides 2.4.1T. Nucleic Acids Res 28: 862-867 [Abstract] [Full Text]  
• Mackenzie, C., Simmons, A. E., Kaplan, S. (1999). Multiple Chromosomes in Bacteria: The Yin and Yang of trp Gene Localization in Rhodobacter sphaeroides 2.4.1. Genetics 153: 525-538 [Abstract] [Full Text]  
• Dubbs, J. M., Tabita, F. R. (1998). Two Functionally Distinct Regions Upstream of the cbbI Operon of Rhodobacter sphaeroides Regulate Gene Expression. J. Bacteriol. 180: 4903-4911 [Abstract] [Full Text]  
• Dubbs, J. M., Bird, T. H., Bauer, C. E., Tabita, F. R. (2000). Interaction of CbbR and RegA* Transcription Regulators with the Rhodobacter sphaeroides cbbIPromoter-Operator Region. J. Biol. Chem. 275: 19224-19230 [Abstract] [Full Text]