This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Boettcher, K J
Right arrow Articles by Ruby, E G
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Boettcher, K J
Right arrow Articles by Ruby, E G

 Previous Article  |  Next Article 

J Bacteriol. 1990 July; 172(7): 3701-3706

research-article

Depressed light emission by symbiotic Vibrio fischeri of the sepiolid squid Euprymna scolopes.

K J Boettcher and E G Ruby

Department of Biological Sciences, University of Southern California, Los Angeles 90089-0371.

ABSTRACT

Bioluminescent marine bacteria of the species Vibrio fischeri are the specific light organ symbionts of the sepiolid squid Euprymna scolopes. Although they share morphological and physiological characteristics with other strains of V. fischeri, when cultured away from the light organ association the E. scolopes symbionts depress their maximal luminescence over 1,000-fold. The primary cause of this reduced luminescence is the underproduction by these bacteria of luciferase autoinducer, a molecule involved in the positive transcriptional regulation of the V. fischeri lux operon. Such an absence of visible light production outside of the symbiotic association has not been previously reported among light organ symbionts of this or any other species of luminous bacteria. Levels of luminescence approaching those of the E. scolopes bacteria in the intact association can be restored by the addition of exogenous autoinducer to bacteria in laboratory culture and are affected by the presence of cyclic AMP. We conclude that some condition(s) specific to the internal environment of the light organ is necessary for maximal autoinduction of luminescence in the symbionts of this squid-bacterial association.

J Bacteriol. 1990 July; 172(7): 3701-3706




This article has been cited by other articles:

  • Adin, D. M., Engle, J. T., Goldman, W. E., McFall-Ngai, M. J., Stabb, E. V. (2009). Mutations in ampG and Lytic Transglycosylase Genes Affect the Net Release of Peptidoglycan Monomers from Vibrio fischeri. J. Bacteriol. 191: 2012-2022 [Abstract] [Full Text]  
  • Learman, D. R., Yi, H., Brown, S. D., Martin, S. L., Geesey, G. G., Stevens, A. M., Hochella, M. F. Jr. (2009). Involvement of Shewanella oneidensis MR-1 LuxS in Biofilm Development and Sulfur Metabolism. Appl. Environ. Microbiol. 75: 1301-1307 [Abstract] [Full Text]  
  • Wollenberg, M. S., Ruby, E. G. (2009). Population Structure of Vibrio fischeri within the Light Organs of Euprymna scolopes Squid from Two Oahu (Hawaii) Populations. Appl. Environ. Microbiol. 75: 193-202 [Abstract] [Full Text]  
  • Lyell, N. L., Dunn, A. K., Bose, J. L., Vescovi, S. L., Stabb, E. V. (2008). Effective Mutagenesis of Vibrio fischeri by Using Hyperactive Mini-Tn5 Derivatives. Appl. Environ. Microbiol. 74: 7059-7063 [Abstract] [Full Text]  
  • Studer, S. V., Mandel, M. J., Ruby, E. G. (2008). AinS Quorum Sensing Regulates the Vibrio fischeri Acetate Switch. J. Bacteriol. 190: 5915-5923 [Abstract] [Full Text]  
  • Dunn, A. K., Stabb, E. V. (2008). Genetic Analysis of Trimethylamine N-Oxide Reductases in the Light Organ Symbiont Vibrio fischeri ES114. J. Bacteriol. 190: 5814-5823 [Abstract] [Full Text]  
  • Darnell, C. L., Hussa, E. A., Visick, K. L. (2008). The Putative Hybrid Sensor Kinase SypF Coordinates Biofilm Formation in Vibrio fischeri by Acting Upstream of Two Response Regulators, SypG and VpsR. J. Bacteriol. 190: 4941-4950 [Abstract] [Full Text]  
  • Antunes, L. C. M., Ferreira, R. B. R., Lostroh, C. P., Greenberg, E. P. (2008). A Mutational Analysis Defines Vibrio fischeri LuxR Binding Sites. J. Bacteriol. 190: 4392-4397 [Abstract] [Full Text]  
  • Geszvain, K., Visick, K. L. (2008). The Hybrid Sensor Kinase RscS Integrates Positive and Negative Signals To Modulate Biofilm Formation in Vibrio fischeri. J. Bacteriol. 190: 4437-4446 [Abstract] [Full Text]  
  • Hussa, E. A., Darnell, C. L., Visick, K. L. (2008). RscS Functions Upstream of SypG To Control the syp Locus and Biofilm Formation in Vibrio fischeri. J. Bacteriol. 190: 4576-4583 [Abstract] [Full Text]  
  • Adin, D. M., Visick, K. L., Stabb, E. V. (2008). Identification of a Cellobiose Utilization Gene Cluster with Cryptic {beta}-Galactosidase Activity in Vibrio fischeri. Appl. Environ. Microbiol. 74: 4059-4069 [Abstract] [Full Text]  
  • Adin, D. M., Phillips, N. J., Gibson, B. W., Apicella, M. A., Ruby, E. G., McFall-Ngai, M. J., Hall, D. B., Stabb, E. V. (2008). Characterization of htrB and msbB Mutants of the Light Organ Symbiont Vibrio fischeri. Appl. Environ. Microbiol. 74: 633-644 [Abstract] [Full Text]  
  • Antunes, L. C. M., Schaefer, A. L., Ferreira, R. B. R., Qin, N., Stevens, A. M., Ruby, E. G., Greenberg, E. P. (2007). Transcriptome Analysis of the Vibrio fischeri LuxR-LuxI Regulon. J. Bacteriol. 189: 8387-8391 [Abstract] [Full Text]  
  • Hussa, E. A., O'Shea, T. M., Darnell, C. L., Ruby, E. G., Visick, K. L. (2007). Two-Component Response Regulators of Vibrio fischeri: Identification, Mutagenesis, and Characterization. J. Bacteriol. 189: 5825-5838 [Abstract] [Full Text]  
  • Visick, K. L., O'Shea, T. M., Klein, A. H., Geszvain, K., Wolfe, A. J. (2007). The Sugar Phosphotransferase System of Vibrio fischeri Inhibits both Motility and Bioluminescence. J. Bacteriol. 189: 2571-2574 [Abstract] [Full Text]  
  • Nelson, E. J., Tunsjo, H. S., Fidopiastis, P. M., Sorum, H., Ruby, E. G. (2007). A Novel lux Operon in the Cryptically Bioluminescent Fish Pathogen Vibrio salmonicida Is Associated with Virulence. Appl. Environ. Microbiol. 73: 1825-1833 [Abstract] [Full Text]  
  • Koropatnick, T. A., Kimbell, J. R., McFall-Ngai, M. J. (2007). Responses of Host Hemocytes During the Initiation of the Squid-Vibrio Symbiosis. Biol. Bull. 212: 29-39 [Abstract] [Full Text]  
  • O'Shea, T. M., Klein, A. H., Geszvain, K., Wolfe, A. J., Visick, K. L. (2006). Diguanylate Cyclases Control Magnesium-Dependent Motility of Vibrio fischeri. J. Bacteriol. 188: 8196-8205 [Abstract] [Full Text]  
  • Walker, E. L., Bose, J. L., Stabb, E. V. (2006). Photolyase Confers Resistance to UV Light but Does Not Contribute to the Symbiotic Benefit of Bioluminescence in Vibrio fischeri ES114. Appl. Environ. Microbiol. 72: 6600-6606 [Abstract] [Full Text]  
  • Goodson, M. S., Crookes-Goodson, W. J., Kimbell, J. R., McFall-Ngai, M. J. (2006). Characterization and Role of p53 Family Members in the Symbiont-Induced Morphogenesis of the Euprymna scolopes Light Organ.. Biol. Bull. 211: 7-17 [Abstract] [Full Text]  
  • Dunn, A. K., Millikan, D. S., Adin, D. M., Bose, J. L., Stabb, E. V. (2006). New rfp- and pES213-Derived Tools for Analyzing Symbiotic Vibrio fischeri Reveal Patterns of Infection and lux Expression In Situ. Appl. Environ. Microbiol. 72: 802-810 [Abstract] [Full Text]  
  • Goodson, M. S., Kojadinovic, M., Troll, J. V., Scheetz, T. E., Casavant, T. L., Soares, M. B., McFall-Ngai, M. J. (2005). Identifying Components of the NF-{kappa}B Pathway in the Beneficial Euprymna scolopes-Vibrio fischeri Light Organ Symbiosis. Appl. Environ. Microbiol. 71: 6934-6946 [Abstract] [Full Text]  
  • Boettcher, K. J., Geaghan, K. K., Maloy, A. P., Barber, B. J. (2005). Roseovarius crassostreae sp. nov., a member of the Roseobacter clade and the apparent cause of juvenile oyster disease (JOD) in cultured Eastern oysters. Int. J. Syst. Evol. Microbiol. 55: 1531-1537 [Abstract] [Full Text]  
  • Lupp, C., Ruby, E. G. (2005). Vibrio fischeri Uses Two Quorum-Sensing Systems for the Regulation of Early and Late Colonization Factors. J. Bacteriol. 187: 3620-3629 [Abstract] [Full Text]  
  • O'Shea, T. M., DeLoney-Marino, C. R., Shibata, S., Aizawa, S.-I., Wolfe, A. J., Visick, K. L. (2005). Magnesium Promotes Flagellation of Vibrio fischeri. J. Bacteriol. 187: 2058-2065 [Abstract] [Full Text]  
  • Ruby, E. G., Urbanowski, M., Campbell, J., Dunn, A., Faini, M., Gunsalus, R., Lostroh, P., Lupp, C., McCann, J., Millikan, D., Schaefer, A., Stabb, E., Stevens, A., Visick, K., Whistler, C., Greenberg, E. P. (2005). Complete genome sequence of Vibrio fischeri: A symbiotic bacterium with pathogenic congeners. Proc. Natl. Acad. Sci. USA 102: 3004-3009 [Abstract] [Full Text]  
  • Lee, J.-H., Rho, J. B., Park, K.-J., Kim, C. B., Han, Y.-S., Choi, S. H., Lee, K.-H., Park, S.-J. (2004). Role of Flagellum and Motility in Pathogenesis of Vibrio vulnificus. Infect. Immun. 72: 4905-4910 [Abstract] [Full Text]  
  • Millikan, D. S., Ruby, E. G. (2004). Vibrio fischeri Flagellin A Is Essential for Normal Motility and for Symbiotic Competence during Initial Squid Light Organ Colonization. J. Bacteriol. 186: 4315-4325 [Abstract] [Full Text]  
  • Lupp, C., Ruby, E. G. (2004). Vibrio fischeri LuxS and AinS: Comparative Study of Two Signal Synthases. J. Bacteriol. 186: 3873-3881 [Abstract] [Full Text]  
  • Stabb, E. V., Butler, M. S., Adin, D. M. (2004). Correlation between Osmolarity and Luminescence of Symbiotic Vibrio fischeri Strain ES114. J. Bacteriol. 186: 2906-2908 [Abstract] [Full Text]  
  • Wolfe, A. J., Millikan, D. S., Campbell, J. M., Visick, K. L. (2004). Vibrio fischeri {sigma}54 Controls Motility, Biofilm Formation, Luminescence, and Colonization. Appl. Environ. Microbiol. 70: 2520-2524 [Abstract] [Full Text]  
  • DeLoney-Marino, C. R., Wolfe, A. J., Visick, K. L. (2003). Chemoattraction of Vibrio fischeri to Serine, Nucleosides, and N-Acetylneuraminic Acid, a Component of Squid Light-Organ Mucus. Appl. Environ. Microbiol. 69: 7527-7530 [Abstract] [Full Text]  
  • McCann, J., Stabb, E. V., Millikan, D. S., Ruby, E. G. (2003). Population Dynamics of Vibrio fischeri during Infection of Euprymna scolopes. Appl. Environ. Microbiol. 69: 5928-5934 [Abstract] [Full Text]  
  • Nyholm, S. V., McFall-Ngai, M. J. (2003). Dominance of Vibrio fischeri in Secreted Mucus outside the Light Organ of Euprymna scolopes: the First Site of Symbiont Specificity. Appl. Environ. Microbiol. 69: 3932-3937 [Abstract] [Full Text]  
  • Millikan, D. S., Ruby, E. G. (2003). FlrA, a {sigma}54-Dependent Transcriptional Activator in Vibrio fischeri, Is Required for Motility and Symbiotic Light-Organ Colonization. J. Bacteriol. 185: 3547-3557 [Abstract] [Full Text]  
  • Stabb, E. V., Ruby, E. G. (2003). Contribution of pilA to Competitive Colonization of the Squid Euprymna scolopes by Vibrio fischeri. Appl. Environ. Microbiol. 69: 820-826 [Abstract] [Full Text]  
  • Nyholm, S. V., Deplancke, B., Gaskins, H. R., Apicella, M. A., McFall-Ngai, M. J. (2002). Roles of Vibrio fischeri and Nonsymbiotic Bacteria in the Dynamics of Mucus Secretion during Symbiont Colonization of the Euprymna scolopes Light Organ. Appl. Environ. Microbiol. 68: 5113-5122 [Abstract] [Full Text]  
  • DeLoney, C. R., Bartley, T. M., Visick, K. L. (2002). Role for Phosphoglucomutase in Vibrio fischeri-Euprymna scolopes Symbiosis. J. Bacteriol. 184: 5121-5129 [Abstract] [Full Text]  
  • Millikan, D. S., Ruby, E. G. (2002). Alterations in Vibrio fischeri Motility Correlate with a Delay in Symbiosis Initiation and Are Associated with Additional Symbiotic Colonization Defects. Appl. Environ. Microbiol. 68: 2519-2528 [Abstract] [Full Text]  
  • Aeckersberg, F., Lupp, C., Feliciano, B., Ruby, E. G. (2001). Vibrio fischeri Outer Membrane Protein OmpU Plays a Role in Normal Symbiotic Colonization. J. Bacteriol. 183: 6590-6597 [Abstract] [Full Text]  
  • Visick, K. L., Skoufos, L. M. (2001). Two-Component Sensor Required for Normal Symbiotic Colonization of Euprymna scolopes by Vibrio fischeri. J. Bacteriol. 183: 835-842 [Abstract] [Full Text]  
  • Stabb, E. V., Reich, K. A., Ruby, E. G. (2001). Vibrio fischeri Genes hvnA and hvnB Encode Secreted NAD+-Glycohydrolases. J. Bacteriol. 183: 309-317 [Abstract] [Full Text]  
  • Indergand, S., Graf, J. (2000). Ingested Blood Contributes to the Specificity of the Symbiosis of Aeromonas veronii Biovar Sobria and Hirudo medicinalis, the Medicinal Leech. Appl. Environ. Microbiol. 66: 4735-4741 [Abstract] [Full Text]  
  • Boettcher, K. J., Barber, B. J., Singer, J. T. (2000). Additional Evidence that Juvenile Oyster Disease Is Caused by a Member of the Roseobacter Group and Colonization of Nonaffected Animals by Stappia stellulata-Like Strains. Appl. Environ. Microbiol. 66: 3924-3930 [Abstract] [Full Text]  
  • Visick, K. L., Foster, J., Doino, J., McFall-Ngai, M., Ruby, E. G. (2000). Vibrio fischeri lux Genes Play an Important Role in Colonization and Development of the Host Light Organ. J. Bacteriol. 182: 4578-4586 [Abstract] [Full Text]  
  • Nishiguchi, M. K. (2000). Temperature Affects Species Distribution in Symbiotic Populations of Vibrio spp.. Appl. Environ. Microbiol. 66: 3550-3555 [Abstract] [Full Text]  
  • Visick, K. L., McFall-Ngai, M. J. (2000). An Exclusive Contract: Specificity in the Vibrio fischeri-Euprymna scolopes Partnership. J. Bacteriol. 182: 1779-1787 [Full Text]  
  • Boettcher, K. J., Barber, B. J., Singer, J. T. (1999). Use of Antibacterial Agents To Elucidate the Etiology of Juvenile Oyster Disease (JOD) in Crassostrea virginica and Numerical Dominance of an alpha -Proteobacterium in JOD-Affected Animals. Appl. Environ. Microbiol. 65: 2534-2539 [Abstract] [Full Text]  
  • Nishiguchi, M. K., Ruby, E. G., McFall-Ngai, M. J. (1998). Competitive Dominance among Strains of Luminous Bacteria Provides an Unusual Form of Evidence for Parallel Evolution in Sepiolid Squid-Vibrio Symbioses. Appl. Environ. Microbiol. 64: 3209-3213 [Abstract] [Full Text]  
  • Visick, K. L., Ruby, E. G. (1998). The Periplasmic, Group III Catalase of Vibrio fischeri Is Required for Normal Symbiotic Competence and Is Induced Both by Oxidative Stress and by Approach to Stationary Phase. J. Bacteriol. 180: 2087-2092 [Abstract] [Full Text]  
  • Ruby, E. G., Lee, K.-H. (1998). The Vibrio fischeri-Euprymna scolopes Light Organ Association: Current Ecological Paradigms. Appl. Environ. Microbiol. 64: 805-812 [Full Text]  
  • Lamarcq, L. H., McFall-Ngai, M. J. (1998). Induction of a Gradual, Reversible Morphogenesis of Its Host's Epithelial Brush Border by Vibrio fischeri. Infect. Immun. 66: 777-785 [Abstract] [Full Text]  
  • Fidopiastis, P. M., Boletzky, S. v., Ruby, E. G. (1998). A New Niche for Vibrio logei, the Predominant Light Organ Symbiont of Squids in the Genus Sepiola. J. Bacteriol. 180: 59-64 [Abstract] [Full Text]  
  • Montgomery, M., McFall-Ngai, M (1994). Bacterial symbionts induce host organ morphogenesis during early postembryonic development of the squid Euprymna scolopes. Development 120: 1719-1729 [Abstract]  
  • McFall-Ngai, M., Ruby, E. (1991). Symbiont recognition and subsequent morphogenesis as early events in an animal-bacterial mutualism. Science 254: 1491-1494 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»