This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wiater, L A Articles by Grindley, N D Search for Related Content PubMed PubMed Citation Articles by Wiater, L A Articles by Grindley, N D

 Previous Article  |  Next Article 

J Bacteriol. 1990 September; 172(9): 4959-4963

research-article

Uncoupling of transpositional immunity from gamma delta transposition by a mutation at the end of gamma delta.

Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06510.

ABSTRACT

The transposon gamma delta, in common with other members of the Tn3 family, confers transpositional immunity, a phenomenon by which plasmids containing a single transposon end show reduced activity as targets for further insertion by the same element. We found that a copy of a mutant delta end, in which the two terminal base pairs (5' GG) were substituted with cytosines, conferred the same degree of immunity as the unaltered delta end. However, a transposon analog with the mutant delta end as its termini could not transpose. These results suggest that the binding of transposase to a site on a target replicon is sufficient to confer immunity and that immunity does not involve subsequent DNA transactions at the bound target site, analogous to the catalytic processes that occur at the transposon ends during transposition.

This article has been cited by other articles:

• Sota, M., Endo, M., Nitta, K., Kawasaki, H., Tsuda, M. (2002). Characterization of a Class II Defective Transposon Carrying Two Haloacetate Dehalogenase Genes from Delftia acidovorans Plasmid pUO1. Appl. Environ. Microbiol. 68: 2307-2315 [Abstract] [Full Text]