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J Bacteriol. 1991 July; 173(13): 3935-3942

research-article

Critical sequences in the core of the P1 plasmid replication origin.

T Brendler, A Abeles and S Austin

Laboratory of Chromosome Biology, NCI-Frederick Cancer Research and Development Center, Maryland 21702.

ABSTRACT

The core of the P1 plasmid replication origin consists of a series of 7-bp repeats and a G+C-rich stretch. Methylation of the GATC sequences in the repeats is essential. Forty different single-base mutations in the region were isolated and assayed for origin function. A single-base change within any 7-bp repeat could block the origin, irrespective of whether GATC bases were affected. The repeats themselves were critical, but the short intervals between them were not. Mutations in the G+C-rich region showed it to be a spacer whose exact length is important but whose sequence can vary considerably. It maintains a precise distance between the 7-bp repeats and binding sites for the P1 RepA initiator protein. It may also serve as a clamp to limit strand separation during initiation.

J Bacteriol. 1991 July; 173(13): 3935-3942




This article has been cited by other articles:

  • Rein, T., Kobayashi, T., Malott, M., Leffak, M., DePamphilis, M. L. (1999). DNA Methylation at Mammalian Replication Origins. J. Biol. Chem. 274: 25792-25800 [Abstract] [Full Text]  
  • Tocchetti, A., Galimberti, G., Dehò, G., Ghisotti, D. (1999). Characterization of the oriI and oriII Origins of Replication in Phage-Plasmid P4. J. Virol. 73: 7308-7316 [Abstract] [Full Text]  
  • del Solar, G., Giraldo, R., Ruiz-Echevarria, M. J., Espinosa, M., Diaz-Orejas, R. (1998). Replication and Control of Circular Bacterial Plasmids. Microbiol. Mol. Biol. Rev. 62: 434-464 [Abstract] [Full Text]  


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