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J Bacteriol. 1991 July; 173(14): 4526-4529

research-article

IS231A from Bacillus thuringiensis is functional in Escherichia coli: transposition and insertion specificity.

B Hallet, R Rezsöhazy and J Delcour

Unité de Génétique, Université Catholique de Louvain, Belgium.

ABSTRACT

A kanamycin resistance gene was introduced within the insertion sequence IS231A from Bacillus thuringiensis, and transposition of the element was demonstrated in Escherichia coli. DNA sequencing at the target sites showed that IS231A transposition results in direct repeats of variable lengths (10, 11, and 12 bp). These target sequences resemble the terminal inverted repeats of the transposon Tn4430, which are the preferred natural insertion sites of IS231 in B. thuringiensis.

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J Bacteriol. 1991 July; 173(14): 4526-4529

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