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Genetic and sequence analyses of a Pseudomonas denitrificans DNA fragment containing two cob genes.

Unité de Biologie Moléculaire, Institut des Biotechnologies, Virty-sur-Seine, France.

ABSTRACT

A genetic analysis of a 12-kb DNA fragment containing Pseudomonas denitrificans cob genes was performed by transposon-mediated insertional mutagenesis. The nucleotide sequence and genetic analysis have shown that a 4.8-kb DNA subfragment carried two cob genes (cobS and cobT). Biochemical data concerning the complemented cobS and cobT mutants suggested that the cobS product was involved in cobalt insertion-mediating reactions and that the cobT product was involved in the transformation of precorrin-3 into cobyrinic acid.

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