This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ruyter, G J Articles by van Dam, K Search for Related Content PubMed PubMed Citation Articles by Ruyter, G J Articles by van Dam, K

research-article

Control of glucose metabolism by enzyme IIGlc of the phosphoenolpyruvate-dependent phosphotransferase system in Escherichia coli.

E. C. Slater Institute for Biochemical Research, University of Amsterdam, The Netherlands.

ABSTRACT

The quantitative effects of variations in the amount of enzyme IIGlc of the phosphoenolpyruvate:glucose phosphotransferase system (PTS) on glucose metabolism in Escherichia coli were studied. The level of enzyme IIGlc could be adjusted in vivo to between 20 and 600% of the wild-type chromosomal level by using the expression vector pTSG11. On this plasmid, expression of the structural gene for enzyme IIGlc, ptsG, is controlled by the tac promoter. As expected, the control coefficient (i.e., the relative increase in pathway flux, divided by the relative increase in amount of enzyme) of enzyme IIGlc decreased in magnitude if a more extensive pathway was considered. Thus, at the wild-type level of enzyme IIGlc activity, the control coefficient of this enzyme on the growth rate on glucose and on the rate of glucose oxidation was low, while the control coefficient on uptake and phosphorylation of methyl alpha-glucopyranoside (an enzyme IIGlc-specific, nonmetabolizable glucose analog) was relatively high (0.55 to 0.65). The implications of our findings for PTS-mediated regulation, i.e., inhibition of growth on non-PTS compounds by glucose, are discussed.

This article has been cited by other articles:

• Becker, A.-K., Zeppenfeld, T., Staab, A., Seitz, S., Boos, W., Morita, T., Aiba, H., Mahr, K., Titgemeyer, F., Jahreis, K. (2006). YeeI, a Novel Protein Involved in Modulation of the Activity of the Glucose-Phosphotransferase System in Escherichia coli K-12.. J. Bacteriol. 188: 5439-5449 [Abstract] [Full Text]  
• Koebmann, B. J., Solem, C., Pedersen, M. B., Nilsson, D., Jensen, P. R. (2002). Expression of Genes Encoding F1-ATPase Results in Uncoupling of Glycolysis from Biomass Production in Lactococcus lactis. Appl. Environ. Microbiol. 68: 4274-4282 [Abstract] [Full Text]  
• Koebmann, B. J., Westerhoff, H. V., Snoep, J. L., Nilsson, D., Jensen, P. R. (2002). The Glycolytic Flux in Escherichia coli Is Controlled by the Demand for ATP. J. Bacteriol. 184: 3909-3916 [Abstract] [Full Text]  
• Solem, C., Jensen, P. R. (2002). Modulation of Gene Expression Made Easy. Appl. Environ. Microbiol. 68: 2397-2403 [Abstract] [Full Text]  
• Zeppenfeld, T., Larisch, C., Lengeler, J. W., Jahreis, K. (2000). Glucose Transporter Mutants of Escherichia coli K-12 with Changes in Substrate Recognition of IICBGlc and Induction Behavior of the ptsG Gene. J. Bacteriol. 182: 4443-4452 [Abstract] [Full Text]  
• Diderich, J. A., Teusink, B., Valkier, J., Anjos, J., Spencer-Martins, I., van Dam, K., Walsh, M. C. (1999). Strategies to determine the extent of control exerted by glucose transport on glycolytic flux in the yeast Saccharomyces bayanus. Microbiology 145: 3447-3454 [Abstract] [Full Text]  
• Bakker, B. M., Walsh, M. C., ter Kuile, B. H., Mensonides, F. I. C., Michels, P. A. M., Opperdoes, F. R., Westerhoff, H. V. (1999). Contribution of glucose transport to the control of the glycolytic flux in Trypanosoma brucei. Proc. Natl. Acad. Sci. USA 96: 10098-10103 [Abstract] [Full Text]  
• Bakker, B. M., Michels, P. A. M., Opperdoes, F. R., Westerhoff, H. V. (1999). What Controls Glycolysis in Bloodstream Form Trypanosoma brucei?. J. Biol. Chem. 274: 14551-14559 [Abstract] [Full Text]  
• Jensen, P. R., Hammer, K. (1998). The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters. Appl. Environ. Microbiol. 64: 82-87 [Abstract] [Full Text]  
• Kimata, K., Takahashi, H., Inada, T., Postma, P., Aiba, H. (1997). cAMP receptor protein-cAMP plays a crucial role in glucose-lactose diauxie by activating the major glucose transporter gene in coli. Proc. Natl. Acad. Sci. USA 94: 12914-12919 [Abstract] [Full Text]  
• Rohwer, J. M., Meadow, N. D., Roseman, S., Westerhoff, H. V., Postma, P. W. (2000). Understanding Glucose Transport by the Bacterial Phosphoenolpyruvate:Glycose Phosphotransferase System on the Basis of Kinetic Measurements in Vitro. J. Biol. Chem. 275: 34909-34921 [Abstract] [Full Text]