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J Bacteriol. 1991 October; 173(19): 6300-6302

research-article

Purification and partial characterization of Cob(I)alamin adenosyltransferase from Pseudomonas denitrificans.

Département Analyse, Centre de Recherche de Vitry-Alfortville, Vitry-sur-Seine, France.

ABSTRACT

Cob(I)alamin adenosyltransferase (EC 2.5.1.17) was purified to homogeneity from extracts of a Pseudomonas denitrificans recombinant strain and sequenced at its N terminus. It is a homodimer (each unit with an Mr of 28,000) encoded by cobO. The enzyme adenosylated all of the corrinoids isolated from this microorganism but did not adenosylate cobyrinic acid.

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