This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Abo, T Articles by Ohtsubo, E Search for Related Content PubMed PubMed Citation Articles by Abo, T Articles by Ohtsubo, E

 Previous Article  |  Next Article 

J Bacteriol. 1991 October; 173(20): 6347-6354

research-article

Specific DNA binding of the TraM protein to the oriT region of plasmid R100.

Institute of Applied Microbiology, University of Tokyo, Japan.

ABSTRACT

The product of the traM gene of plasmid R100 was purified as the TraM-collagen-beta-galactosidase fusion protein (TraM*) by using a beta-galactosidase-specific affinity column, and the TraM portion of TraM* (TraM') was separated by collagenolysis. Both the TraM* and TraM' proteins were found to bind specifically to a broad region preceding the traM gene. This region (designated sbm) was located within the nonconserved region in oriT among conjugative plasmids related to R100. The region seems to contain four core binding sites (designated sbmA, sbmB, sbmC, and sbmD), each consisting of a similar number of nucleotides and including a homologous 15-bp sequence. This result, together with the observation that the TraM* protein was located in the membrane fraction, indicates the possibility that the TraM protein has a function in anchoring the oriT region of R100 at the sbm sites to the membrane pore, through which the single-stranded DNA is transferred to the recipient. sbmC and sbmD, each of which contained a characteristic inverted repeat sequence, overlapped with the promoter region for the traM gene. This suggests that the expression of the traM gene may be regulated by its own product.

This article has been cited by other articles:

• Matson, S. W., Ragonese, H. (2005). The F-Plasmid TraI Protein Contains Three Functional Domains Required for Conjugative DNA Strand Transfer. J. Bacteriol. 187: 697-706 [Abstract] [Full Text]  
• Beranek, A., Zettl, M., Lorenzoni, K., Schauer, A., Manhart, M., Koraimann, G. (2004). Thirty-Eight C-Terminal Amino Acids of the Coupling Protein TraD of the F-Like Conjugative Resistance Plasmid R1 Are Required and Sufficient To Confer Binding to the Substrate Selector Protein TraM. J. Bacteriol. 186: 6999-7006 [Abstract] [Full Text]  
• Miller, D. L., Schildbach, J. F. (2003). Evidence for a Monomeric Intermediate in the Reversible Unfolding of F Factor TraM. J. Biol. Chem. 278: 10400-10407 [Abstract] [Full Text]  
• Karl, W., Bamberger, M., Zechner, E. L. (2001). Transfer Protein TraY of Plasmid R1 Stimulates TraI-Catalyzed oriT Cleavage In Vivo. J. Bacteriol. 183: 909-914 [Abstract] [Full Text]  
• Fekete, R. A., Frost, L. S. (2000). Mobilization of Chimeric oriT Plasmids by F and R100-1: Role of Relaxosome Formation in Defining Plasmid Specificity. J. Bacteriol. 182: 4022-4027 [Abstract] [Full Text]