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J Bacteriol. 1991 December; 173(23): 7728-7731

research-article

Examination of the phosphate in conjugative F-like pili by use of electron spectroscopic imaging.

L S Frost and D P Bazett-Jones

Department of Microbiology, University of Alberta, Edmonton, Canada.

ABSTRACT

F-like pili specified by conjugative plasmids have been reported to contain phosphate which may be noncovalently incorporated into the pilus. Electron spectroscopic imaging was able to detect phosphate in the filamentous, single-stranded DNA phage f1, used as positive control, but could not detect phosphate in F-like pili. Thus, the phosphate in purified pili which has been reported is probably derived from contaminating cell envelope material.


J Bacteriol. 1991 December; 173(23): 7728-7731