This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Haney, S A
Right arrow Articles by Calvo, J M
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Haney, S A
Right arrow Articles by Calvo, J M

 Previous Article  |  Next Article 

J Bacteriol. 1992 January; 174(1): 108-115

research-article

Lrp, a leucine-responsive protein, regulates branched-chain amino acid transport genes in Escherichia coli.

S A Haney, J V Platko, D L Oxender and J M Calvo

Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor 48109-0606.

ABSTRACT

We investigated the relationship between two regulatory genes, livR and lrp, that map near min 20 on the Escherichia coli chromosome. livR was identified earlier as a regulatory gene affecting high-affinity transport of branched-chain amino acids through the LIV-I and LS transport systems, encoded by the livJ and livKHMGF operons. lrp was characterized more recently as a regulatory gene of a regulon that includes operons involved in isoleucine-valine biosynthesis, oligopeptide transport, and serine and threonine catabolism. The expression of each of these livR- and lrp-regulated operons is altered in cells when leucine is added to their growth medium. The following results demonstrate that livR and lrp are the same gene. The lrp gene from a livR1-containing strain was cloned and shown to contain two single-base-pair substitutions in comparison with the wild-type strain. Mutations in livR affected the regulation of ilvIH, an operon known to be controlled by lrp, and mutations in lrp affected the regulation of the LIV-I and LS transport systems. Lrp from a wild-type strain bound specifically to several sites upstream of the ilvIH operon, whereas binding by Lrp from a livR1-containing strain was barely detectable. In a strain containing a Tn10 insertion in lrp, high-affinity leucine transport occurred at a high, constitutive level, as did expression from the livJ and livK promoters as measured by lacZ reporter gene expression. Taken together, these results suggest that Lrp acts directly or indirectly to repress livJ and livK expression and that leucine is required for this repression. This pattern of regulation is unusual for operons that are controlled by Lrp.

J Bacteriol. 1992 January; 174(1): 108-115




This article has been cited by other articles:

  • Cho, B.-K., Barrett, C. L., Knight, E. M., Park, Y. S., Palsson, B. O. (2008). Genome-scale reconstruction of the Lrp regulatory network in Escherichia coli. Proc. Natl. Acad. Sci. USA 105: 19462-19467 [Abstract] [Full Text]  
  • Park, J. H., Lee, K. H., Kim, T. Y., Lee, S. Y. (2007). Metabolic engineering of Escherichia coli for the production of L-valine based on transcriptome analysis and in silico gene knockout simulation. Proc. Natl. Acad. Sci. USA 104: 7797-7802 [Abstract] [Full Text]  
  • Berthiaume, F., Crost, C., Labrie, V., Martin, C., Newman, E. B., Harel, J. (2004). Influence of L-Leucine and L-Alanine on Lrp Regulation of foo, Coding for F1651, a Pap Homologue. J. Bacteriol. 186: 8537-8541 [Abstract] [Full Text]  
  • Koyanagi, T., Katayama, T., Suzuki, H., Kumagai, H. (2004). Identification of the LIV-I/LS System as the Third Phenylalanine Transporter in Escherichia coli K-12. J. Bacteriol. 186: 343-350 [Abstract] [Full Text]  
  • Hung, S.-p., Baldi, P., Hatfield, G. W. (2002). Global Gene Expression Profiling in Escherichia coli K12. THE EFFECTS OF LEUCINE-RESPONSIVE REGULATORY PROTEIN. J. Biol. Chem. 277: 40309-40323 [Abstract] [Full Text]  
  • Tani, T. H., Khodursky, A., Blumenthal, R. M., Brown, P. O., Matthews, R. G. (2002). Adaptation to famine: A family of stationary-phase genes revealed by microarray analysis. Proc. Natl. Acad. Sci. USA 99: 13471-13476 [Abstract] [Full Text]  
  • Kennerknecht, N., Sahm, H., Yen, M.-R., Patek, M., Saier, M. H. Jr., Eggeling, L. (2002). Export of L-Isoleucine from Corynebacterium glutamicum: a Two-Gene-Encoded Member of a New Translocator Family. J. Bacteriol. 184: 3947-3956 [Abstract] [Full Text]  
  • Dorazi, R., Dewar, S. J. (2000). The SOS promoter dinH is essential for ftsK transcription during cell division. Microbiology 146: 2891-2899 [Abstract] [Full Text]  
  • Landgraf, J. R., Boxer, J. A., Calvo, J. M. (1999). Escherichia coli Lrp (Leucine-Responsive Regulatory Protein) Does Not Directly Regulate Expression of the leu Operon Promoter. J. Bacteriol. 181: 6547-6551 [Abstract] [Full Text]  
  • Janes, B. K., Bender, R. A. (1999). Two Roles for the Leucine-Responsive Regulatory Protein in Expression of the Alanine Catabolic Operon (dadAB) in Klebsiella aerogenes. J. Bacteriol. 181: 1054-1058 [Abstract] [Full Text]  
  • Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]  
  • Rhee, K. Y., Parekh, B. S., Hatfield, G. W. (1996). Leucine-responsive Regulatory Protein-DNA Interactions in the Leader Region of the ilvGMEDA Operon of Escherichia coli. J. Biol. Chem. 271: 26499-26507 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»